Kascsak R J, Rubenstein R, Merz P A, Carp R I, Robakis N K, Wisniewski H M, Diringer H
J Virol. 1986 Sep;59(3):676-83. doi: 10.1128/JVI.59.3.676-683.1986.
Scrapie-associated fibrils (SAFs) are abnormal filamentous structures that are uniquely associated with unconventional slow virus diseases. The antigenic relationships of SAFs from animals infected with four biologically distinct scrapie strains were investigated by using antisera raised to purified SAF proteins. Rabbit antisera were raised to SAFs isolated from mice infected with the ME7 scrapie strain and to SAFs isolated from hamsters infected with the 263K scrapie strain. A strong antigenic relationship was shown among SAF proteins (PrPs) isolated from all scrapie-infected animals (ME7, 139A, and 87V in mice and 263K in hamsters), and this relationship was demonstrable regardless of which antiserum was used. SAF proteins were antigenically distinct from those of paired helical filaments or amyloid isolated from patients with Alzheimer disease. Distinct Western blot profiles were demonstrated for SAFs isolated from animals infected with each scrapie strain. Differences seen among SAFs were independent, at least in part, of host species or genotype, implying that certain specific structural and molecular properties of SAFs are mediated by the strain of scrapie agent.
瘙痒病相关纤维（SAFs）是异常的丝状结构，与非传统性慢病毒疾病有独特关联。通过使用针对纯化的SAF蛋白产生的抗血清，研究了感染四种生物学上不同的瘙痒病毒株的动物来源SAFs的抗原关系。用从感染ME7瘙痒病毒株的小鼠中分离的SAFs以及从感染263K瘙痒病毒株的仓鼠中分离的SAFs制备兔抗血清。从所有感染瘙痒病的动物（小鼠中的ME7、139A和87V以及仓鼠中的263K）中分离的SAF蛋白（PrPs）之间显示出强烈的抗原关系，并且无论使用哪种抗血清，这种关系都可得到证实。SAF蛋白在抗原性上与从阿尔茨海默病患者分离的成对螺旋丝或淀粉样蛋白不同。从感染每种瘙痒病毒株的动物中分离的SAFs显示出不同的蛋白质印迹图谱。SAFs之间的差异至少部分独立于宿主物种或基因型，这意味着SAFs的某些特定结构和分子特性是由瘙痒病病原体毒株介导的。
