Protein Misfolding Disorders Laboratory, Mitchell Center for Alzheimer's Disease and Related Brain Disorders, University of Texas Medical School at Houston, Houston, TX 77030, USA; Department of Laboratory Animal Science, School of Basic Medical Science, Capital Medical University, Beijing 100069, China.
Protein Misfolding Disorders Laboratory, Mitchell Center for Alzheimer's Disease and Related Brain Disorders, University of Texas Medical School at Houston, Houston, TX 77030, USA.
FEBS Lett. 2014 Mar 3;588(5):795-800. doi: 10.1016/j.febslet.2014.01.038. Epub 2014 Feb 1.
The mechanisms implicated in prion infection and tissue distribution are not completely understood. In this study we investigated the levels of 263K prions in brain and spleen of Syrian hamsters few days after intra-peritoneal challenge. For this purpose we utilized the PMCA technology which permits to detect as little as few PrP(Sc) molecules. Our results show that peripherally administered prions directly reach the brain, although at levels below the minimum necessary to produce disease. PrP(Sc) remains in the brain several days after administration suggesting inefficient clearance or early replication. Understanding the fate of the infectious agent after administration and its uptake in different organs and fluids may provide useful information to develop strategies to minimize further spreading of prion diseases.
朊病毒感染和组织分布的机制尚不完全清楚。在这项研究中，我们研究了腹腔内 challenged 几天后叙利亚仓鼠大脑和脾脏中 263K 朊病毒的水平。为此，我们利用 PMCA 技术，该技术可以检测到很少的 PrP(Sc)分子。我们的结果表明，外周给予的朊病毒直接到达大脑，尽管水平低于产生疾病所需的最低水平。给予后 PrP(Sc)在大脑中持续存在数天，表明清除效率低下或早期复制。了解给药后感染因子的命运及其在不同器官和液体中的摄取，可能为制定策略以最大程度减少朊病毒病的进一步传播提供有用信息。
