Kulikov S N, Chirkov S N, Il'ina A V, Lopatin S A, Varlamov V P
Prikl Biokhim Mikrobiol. 2006 Mar-Apr;42(2):224-8.
The effect of the molecular weight of chitosan on its ability to suppress systemic infection of bean mild mosaic virus in bean (Phasoleus vulgaris L.) plants was studied. The enzymatic hydrolysate of low-molecular-weight chitosan was successively fractionated by ultrafiltration through membranes with decreasing pore size. In total, four chitosan fractions with a weight-average molecular weight varying from 1.2 to 40.4 kDa were obtained. It was shown that the treatments of bean plants with these fractions (chitosan concentration, 10 or 100 microg/ml) inhibited virus accumulation and systemic propagation. The degree of chitosan-induced antiviral resistance increased as the molecular weight of chitosan decreased. The monomers comprising the chitosan molecule-glucosamine and N-acetylglucosamine--exhibited no antiviral activity.
研究了壳聚糖分子量对其抑制菜豆普通花叶病毒在菜豆（Phaseolus vulgaris L.）植株上系统感染能力的影响。低分子量壳聚糖的酶解产物通过孔径逐渐减小的膜进行超滤分级。总共获得了四个重均分子量在1.2至40.4 kDa之间变化的壳聚糖级分。结果表明，用这些级分处理菜豆植株（壳聚糖浓度为10或100μg/ml）可抑制病毒积累和系统传播。壳聚糖诱导的抗病毒抗性程度随着壳聚糖分子量的降低而增加。构成壳聚糖分子的单体——葡萄糖胺和N - 乙酰葡萄糖胺——没有抗病毒活性。
