Il'ina A V, Kulikov S N, Chalenko G I, Gerasimova N G, Varlamov V P
Prikl Biokhim Mikrobiol. 2008 Sep-Oct;44(5):606-14.
The possibility of obtaining monosaccharide derivatives of low-molecular-weight chitosan with the use of the Maillard reaction was studied. Chitosan derivatives (molecular weight, 24 and 5 kDa) obtained with glucosamine, N-acetyl galactosamine, galactose, and mannose with a substitution degree of 4-14% and a yield of 60-80% were obtained. Some physicochemical and biological properties of these derivatives were studied. We showed that monosaccharide derivatives of low-molecular-weight chitosan exhibited antibacterial activity. Chitosan at a concentration of 0.01% caused 100% death of bacteria B. subtilis and E. coil. The strongest antibacterial effect was exhibited by 24-kDa derivatives: only 0.02-0.08% of cells survived. These derivatives were two orders of magnitude more effective than the 5-kDa chitosan modified with galactose.
研究了利用美拉德反应获得低分子量壳聚糖单糖衍生物的可能性。得到了壳聚糖衍生物（分子量分别为24 kDa和5 kDa），其由葡糖胺、N - 乙酰半乳糖胺、半乳糖和甘露糖制得，取代度为4 - 14%，产率为60 - 80%。研究了这些衍生物的一些物理化学和生物学性质。我们发现低分子量壳聚糖的单糖衍生物具有抗菌活性。浓度为0.01%的壳聚糖可导致枯草芽孢杆菌和大肠杆菌100%死亡。24 kDa的衍生物表现出最强的抗菌效果：只有0.02 - 0.08%的细胞存活。这些衍生物的效果比用半乳糖修饰的5 kDa壳聚糖高两个数量级。
