Huang Kuo-Ting, Wu Bing-Ching, Lin Chang-Ching, Luo Shih-Chi, Chen Chinpan, Wong Chi-Huey, Lin Chun-Cheng
Institute of Chemistry and Genomic Research Center, Academia Sinica, Taipei 115, Taiwan.
Carbohydr Res. 2006 Sep 4;341(12):2151-5. doi: 10.1016/j.carres.2006.04.047. Epub 2006 Jun 9.
An enzymatic one-pot three-step glycosylation strategy was developed for the synthesis of sLex moiety of truncated PSGL-1 glycopeptide with and without sulfation. The method provided an efficient way to afford complex glycopeptides in a semi-preparative scale without further complicated and time-consuming purification process in each glycosylation step.
开发了一种酶促一锅三步糖基化策略，用于合成具有和不具有硫酸化的截短PSGL-1糖肽的sLex部分。该方法提供了一种有效的途径，能够以半制备规模获得复杂的糖肽，且在每个糖基化步骤中无需进一步复杂且耗时的纯化过程。
