Costas E, Lopez-Rodas V
Genética, Producción Animal, Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, Spain.
Water Res. 2006 Jul;40(12):2447-51. doi: 10.1016/j.watres.2006.04.013. Epub 2006 Jun 8.
Works correlating fluctuating asymmetry with environmental stress or genetic damages have been largely reported in multicellular organisms but not in single-celled ones. We hypothesize that asymmetry analysis could also be applied to single-celled organisms, because the asymmetry between two sister cells originated from a cellular division event (same genotype in similar environment) must tend to zero in the absence of environmental or genetic perturbations. Laboratory experiments with copper sulphate and DCMU-herbicide treatments as well as experiments in a water reservoir after treatment with copper sulphate algaecide show that environmental stress increases asymmetry between sister cells of Microcystis aeruginosa (Cyanobacteria). Even low Cu(2+) or DCMU doses, which were unable to reduce growth rate, considerably enlarge asymmetry with respect to untreated controls. Asymmetry between sister cells of cyanobacteria seems to be a reliable indicator of environmental perturbation. Analysis of asymmetry in single-celled organisms could become as important as fluctuating asymmetry of multicellular organisms is today.
在多细胞生物中，大量研究报告了波动不对称性与环境压力或基因损伤之间的关联，但在单细胞生物中却鲜有报道。我们推测，不对称性分析也可应用于单细胞生物，因为在没有环境或基因干扰的情况下，源于细胞分裂事件（相似环境中的相同基因型）的两个姐妹细胞之间的不对称性必然趋于零。使用硫酸铜和二氯苯基二甲基脲除草剂处理的实验室实验，以及在使用硫酸铜除藻剂处理后的水库中进行的实验表明，环境压力会增加铜绿微囊藻（蓝细菌）姐妹细胞之间的不对称性。即使是低剂量的铜离子或二氯苯基二甲基脲，虽然无法降低生长速率，但与未处理的对照组相比，也会显著增大不对称性。蓝细菌姐妹细胞之间的不对称性似乎是环境扰动的可靠指标。对单细胞生物不对称性的分析可能会变得与如今多细胞生物的波动不对称性分析一样重要。
