San Miguel Jesús F, Gutiérrez Norma C, Mateo Gema, Orfao Alberto
Servicio de Haematología, Hospital Universitario de Salamanca, Paseo de San Vicente 58-182, 37007, Salamanca, Spain.
Eur J Cancer. 2006 Jul;42(11):1510-9. doi: 10.1016/j.ejca.2005.11.039. Epub 2006 Jun 9.
This paper reviews the most relevant laboratory techniques currently used for the evaluation of patients with multiple myeloma (MM) and other monoclonal gammopathies. Although the bone marrow morphological examination and electrophoretic analysis of the monoclonal paraprotein still remain the 'gold standard' techniques for fast, accurate and cost-effective diagnosis, other assays such as immunophenotyping, DNA cell content and cell cycle analysis measured by flow cytometry may contribute to a better assessment of myeloma patients. Here, we will discuss not only the contribution of each technique to differential diagnosis of monoclonal gammopathies, but also the value of each parameter as prognostic factor and for monitoring treatment efficacy. In addition, possible technical pitfalls inherent to each technique will be analysed.
本文综述了目前用于评估多发性骨髓瘤（MM）和其他单克隆丙种球蛋白病患者的最相关实验室技术。尽管骨髓形态学检查和单克隆副蛋白的电泳分析仍是快速、准确且经济高效诊断的“金标准”技术，但其他检测方法，如通过流式细胞术进行的免疫表型分析、DNA细胞含量和细胞周期分析，可能有助于更好地评估骨髓瘤患者。在此，我们不仅将讨论每种技术对单克隆丙种球蛋白病鉴别诊断的贡献，还将讨论每个参数作为预后因素和监测治疗疗效的价值。此外，还将分析每种技术固有的可能技术缺陷。
