Weecharangsan Wanlop, Opanasopit Praneet, Sukma Monrudee, Ngawhirunpat Tanasait, Sotanaphun Uthai, Siripong Pongpan
Faculty of Pharmacy, Silpakorn University, Nakhonpathom, Thailand.
Med Princ Pract. 2006;15(4):281-7. doi: 10.1159/000092991.
The aim of this study was to investigate the antioxidative and neuroprotective activities of various extracts from the fruit hull of mangosteen (Garcinia mangostana Linn., GM).
Four extracts: water, 50% ethanol, 95% ethanol and ethyl acetate, were used. The antioxidative activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging assay at extract concentrations of 1, 10, 50 and 100 microg/ml. Based on the free radical scavenging activity of the extracts, two (water and 50% ethanol) were selected for their protective activity in NG108-15 neuroblastoma cells against H(2)O(2)-induced oxidative stress and for cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
All extracts exhibited antioxidative activity. The water and 50% ethanol extracts showed high free-radical scavenging activity with IC(50) values of 34.98 +/- 2.24 and 30.76 +/- 1.66 microg/ml, respectively. Both water and 50% ethanol extracts exhibited neuroprotective activity on NG108-15 cells. The highest activity was observed at the concentration of 50 microg/ml for both the water and 50% ethanol extracts. For cytotoxicity test, none of the extracts was toxic to the cells except at the high concentration of 100 microg/ml.
These results suggest that the water and 50% ethanol extracts from the fruit hull of GM may be potent neuroprotectants.
本研究旨在探讨山竹果壳（莽吉柿，Garcinia mangostana Linn.，GM）不同提取物的抗氧化和神经保护活性。
使用四种提取物：水提取物、50%乙醇提取物、95%乙醇提取物和乙酸乙酯提取物。在提取物浓度为1、10、50和100μg/ml时，采用2,2-二苯基-1-苦基肼自由基清除法评估抗氧化活性。基于提取物的自由基清除活性，选择两种提取物（水提取物和50%乙醇提取物），通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法检测其对NG108-15神经母细胞瘤细胞抗H₂O₂诱导的氧化应激的保护活性及细胞活力。
所有提取物均表现出抗氧化活性。水提取物和50%乙醇提取物显示出较高的自由基清除活性，IC₅₀值分别为34.98±2.24和30.76±1.66μg/ml。水提取物和50%乙醇提取物对NG108-15细胞均表现出神经保护活性。水提取物和50%乙醇提取物在浓度为50μg/ml时均观察到最高活性。对于细胞毒性试验，除高浓度100μg/ml外，所有提取物对细胞均无毒性。
这些结果表明，GM果壳的水提取物和50%乙醇提取物可能是有效的神经保护剂。
