Orbea Amaia, Cajaraville Miren P
Biologia Zelularra eta Histologia Laborategia, Zoologia eta Biologia Zelularra Saila, Zientzia eta Teknologia Fakultatea, Euskal Herriko Unibertsitatea/Universidad del Pafs Vasco, 644 P.K., E-48080 Bilbo, Basque Country, Spain.
Environ Toxicol Chem. 2006 Jun;25(6):1616-26. doi: 10.1897/04-520r2.1.
We aimed to determine the effects of changes in polycyclic aromatic hydrocarbon (PAH) and polychlorinated biphenyl (PCB) bioavailability on peroxisome proliferation and antioxidant enzymes to assess their potential use as pollution biomarkers. For this, mussels (Mytilus galloprovincialis) were sampled in Txatxarramendi and transplanted to Arriluze, Muskiz, and Plentzia (northern Spain), and vice versa. Arriluze was the most heavily polluted site, followed by Plentzia, Txatxarramendi, and Muskiz. Animals transplanted from a polluted station to a cleaner station lost contaminant load in three to six weeks, and when transplanted to a more polluted station, they accumulated PAHs and PCBs accordingly. Peroxisomal acyl-A oxidase (AOX) activity was the highest in animals from Arriluze, and animals transplanted to Arriluze showed increased AOX activity, reaching the levels of native mussels. Mussels from Txatxarramendi showed the lowest peroxisomal volume density (Vvp). Among mussels from Txatxarramendi, only those transplanted to Plentzia showed increased Vvp, whereas animals from Arriluze and Muskiz transplanted to Txatxarramendi after 2 d and six weeks, respectively, had decreased Vvp. Two days after transplant, superoxide dismutase and glutathione peroxidase activities were significantly lower in mussels from Arriluze and Muskiz, respectively, than in those from Txatxarramendi and than in animals transplanted to Txatxarramendi from these stations. In conclusion, transplant experiments with mussels are useful to assess the effects of pollution. Peroxisome proliferation, measured as induction of AOX or increased Vvp, shows great potential as a biomarker of pollution to be included in pollution monitoring programs. In the present work, we have demonstrated that peroxisome proliferation is a reversible phenomenon in mussels.
我们旨在确定多环芳烃（PAH）和多氯联苯（PCB）生物可利用性的变化对过氧化物酶体增殖和抗氧化酶的影响，以评估它们作为污染生物标志物的潜在用途。为此，在Txatxarramendi采集贻贝（Mytilus galloprovincialis）并移植到西班牙北部的Arriluze、Muskiz和Plentzia，反之亦然。Arriluze是污染最严重的地点，其次是Plentzia、Txatxarramendi和Muskiz。从污染站点移植到清洁站点的动物在三到六周内污染物负荷降低，而移植到污染更严重站点的动物则相应地积累了多环芳烃和多氯联苯。过氧化物酶体酰基辅酶A氧化酶（AOX）活性在来自Arriluze的动物中最高，移植到Arriluze的动物AOX活性增加，达到本地贻贝的水平。来自Txatxarramendi的贻贝过氧化物酶体体积密度（Vvp）最低。在来自Txatxarramendi的贻贝中，只有那些移植到Plentzia的贻贝Vvp增加，而分别在2天和六周后从Arriluze和Muskiz移植到Txatxarramendi的动物Vvp降低。移植两天后，来自Arriluze和Muskiz的贻贝中超氧化物歧化酶和谷胱甘肽过氧化物酶活性分别显著低于来自Txatxarramendi的贻贝以及从这些站点移植到Txatxarramendi的动物。总之，贻贝移植实验有助于评估污染的影响。以AOX诱导或Vvp增加来衡量的过氧化物酶体增殖作为污染生物标志物在污染监测计划中具有很大潜力。在本研究中，我们证明了过氧化物酶体增殖在贻贝中是一种可逆现象。
