Su Wen-Ta, Liao Yung-Feng, Chu I-Ming
Department of Chemical Engineering, National Taipei University of Technology, Taipei 106, Taiwan.
Micron. 2007;38(3):278-85. doi: 10.1016/j.micron.2006.04.008. Epub 2006 May 15.
We used a hydrophobic micro-textured poly-dimethylsiloxane (PDMS) in the presence of serum protein at 37 degrees C to study the motility of mouse stromal fibroblast on variant (15-100microm) parallel ridge/groove with 30microm depth. In this paper, we observed the temporal changes in cell morphology and locomotion by using time-lapse phase-contrast microscopy. When fibroblasts seeded onto the micro-grooved substrate, almost all of cells concentrated at the bottom of the grooves. Sequentially, the fibroblasts attached and spread on the surface, migrated toward the walls of the grooves, climbed up and down the ridges frequently, apparently, the 30microm depth of groove did not hinder movement across the micro-grooves. Eventually, they stopped proliferating as a result of contact inhibition and formed a confluent monolayer on the ridges almost exclusively, with an orientation parallel to the direction of the ridge/groove. Cellular shape of fibroblast was enhanced with the micro-grooves, the form index of nucleus was 2.6-fold greater than that of cells on smooth surfaces. Further, we found that hydrophobic surfaces are more prone to direct cellular motility in comparison with hydrophilic surfaces.
我们在37摄氏度的血清蛋白存在下，使用疏水性微纹理聚二甲基硅氧烷（PDMS）来研究小鼠基质成纤维细胞在深度为30微米、间距为15 - 100微米的平行脊/槽上的运动性。在本文中，我们通过延时相差显微镜观察了细胞形态和运动的时间变化。当成纤维细胞接种到微槽基底上时，几乎所有细胞都聚集在槽底部。随后，成纤维细胞附着并铺展在表面，向槽壁迁移，频繁地在脊上爬上爬下，显然，30微米深的槽并未阻碍细胞穿过微槽。最终，它们因接触抑制而停止增殖，几乎仅在脊上形成汇合的单层，其取向与脊/槽方向平行。微槽增强了成纤维细胞的细胞形态，细胞核的形态指数比光滑表面上的细胞大2.6倍。此外，我们发现与亲水性表面相比，疏水性表面更易于引导细胞运动。
