Albrecht-Buehler G
Cell Motil Cytoskeleton. 1987;7(1):54-67. doi: 10.1002/cm.970070108.
In order to analyze the cellular mechanisms of shape formation, the shape of individual 3T3 cells was perturbed by micromanipulation resulting in the detachment and relaxation of a cellular extension and the bending of the extension to form an "elbow" at a variable angle beta. Finally, the tip of the extension was allowed to reattach to the substrate away from the cell. The cells reacted by drawing the extension tight. If beta less than 90 degrees, the elbow moved laterally for 8-15 min until the extension projected orthogonally at the cell surface. If beta greater than or equal to 90 degrees, the extension remained stationary. Finally, in all cases webs formed between attachment points in the perturbed area. If the tip of the extension was allowed to touch its own cell body, thus forming a loop, the cells invariably closed the loop. The paper interprets the cellular reaction as the result of cortical tension and suggests that it is a major factor in the formation of fibroblast shape and the expressions of fibroblast motility.
为了分析细胞形态形成的细胞机制，通过显微操作扰动单个3T3细胞的形态，导致细胞突起的分离和松弛，以及突起弯曲形成一个角度可变的β的“肘部”。最后，使突起的尖端在远离细胞的位置重新附着于底物。细胞通过拉紧突起做出反应。如果β小于90度，肘部横向移动8 - 15分钟，直到突起在细胞表面正交伸出。如果β大于或等于90度，突起保持静止。最后，在所有情况下，在扰动区域的附着点之间形成网。如果让突起的尖端接触其自身的细胞体，从而形成一个环，细胞总是会闭合这个环。该论文将细胞反应解释为皮质张力的结果，并表明它是成纤维细胞形态形成和成纤维细胞运动性表达的主要因素。
