Lee Kwangwon, Singh Pratibha, Chung Wen-Chuan, Ash Joshua, Kim Tae Sung, Hang Lisa, Park Sohyun
Department of Plant Pathology, Cornell University, Ithaca, NY 14850, USA.
Fungal Genet Biol. 2006 Oct;43(10):694-706. doi: 10.1016/j.fgb.2006.04.005. Epub 2006 Jun 9.
Light is a major environmental factor that influences many biological processes. We characterized the roles of light in asexual development (including the formation of aerial hyphae and conidiophore) in Magnaporthe oryzae, which is the causal agent of rice blast disease. Our data revealed a complex nature of light regulation in the asexual developments of M. oryzae. Asexual development of M. oryzae is suppressed by blue light in a light/dark cycling environment and asexual spore release is controlled by both blue and red light. We demonstrated that even very dim light, about 10 micromol m(-2), is sufficient to suppress spore-release behavior in M. oryzae. We also generated knockout strains of a blue light receptor, mgwc-1, the M. oryzae homolog of white collar-1 in Neurospora crassa, and demonstrated blue-light-specific regulation in the asexual development and spore release in M. oryzae. Our findings in this agriculturally important pathogen, M. oryzae, broaden our understanding of the roles of light in fungal development.
光是影响许多生物过程的主要环境因素。我们阐述了光在稻瘟病菌无性发育（包括气生菌丝和分生孢子梗的形成）中的作用，稻瘟病菌是水稻稻瘟病的病原体。我们的数据揭示了稻瘟病菌无性发育中光调节的复杂性。在光/暗循环环境中，蓝光抑制稻瘟病菌的无性发育，而无性孢子释放受蓝光和红光共同控制。我们证明，即使是非常微弱的光，约10微摩尔·米-2，也足以抑制稻瘟病菌的孢子释放行为。我们还构建了蓝光受体mgwc-1的敲除菌株，mgwc-1是粗糙脉孢菌白领-1在稻瘟病菌中的同源物，并证明了蓝光对稻瘟病菌无性发育和孢子释放的特异性调节。我们在这种具有重要农业意义的病原体——稻瘟病菌中的发现，拓宽了我们对光在真菌发育中作用的理解。
