Ueno Morio, Matsumura Michiru, Watanabe Kiichi, Nakamura Takahiro, Osakada Fumitaka, Takahashi Masayo, Kawasaki Hiroshi, Kinoshita Shigeru, Sasai Yoshiki
Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo, Kobe 650-0047, Japan.
Proc Natl Acad Sci U S A. 2006 Jun 20;103(25):9554-9. doi: 10.1073/pnas.0600104103. Epub 2006 Jun 9.
Here we report a human-derived material with potent inductive activity that selectively converts ES cells into neural tissues. Both mouse and human ES cells efficiently differentiate into neural precursors when cultured on the matrix components of the human amniotic membrane in serum-free medium [amniotic membrane matrix-based ES cell differentiation (AMED)]. AMED-induced neural tissues have regional characteristics (brainstem) similar to those induced by coculture with mouse PA6 stromal cells [a common method called stromal cell-derived inducing activity (SDIA) culture]. Like the SDIA culture, the AMED system is applicable to the in vitro generation of various CNS tissues, including dopaminergic neurons, motor neurons, and retinal pigment epithelium. In contrast to the SDIA method, which uses animal cells, the AMED culture uses a noncellular inductive material derived from an easily available human tissue; therefore, AMED should provide a more suitable and versatile system for generating a variety of neural tissues for clinical applications.
在此，我们报告了一种具有强大诱导活性的人源材料，它能选择性地将胚胎干细胞转化为神经组织。当在无血清培养基中培养于人类羊膜的基质成分上时，小鼠和人类胚胎干细胞都能高效分化为神经前体细胞[基于羊膜基质的胚胎干细胞分化（AMED）]。AMED诱导产生的神经组织具有与通过与小鼠PA6基质细胞共培养诱导产生的神经组织（一种称为基质细胞衍生诱导活性（SDIA）培养的常用方法）相似的区域特征（脑干）。与SDIA培养一样，AMED系统适用于体外生成各种中枢神经系统组织，包括多巴胺能神经元、运动神经元和视网膜色素上皮。与使用动物细胞的SDIA方法不同，AMED培养使用的是一种源自易于获取的人类组织的无细胞诱导材料；因此，AMED应该为临床应用生成各种神经组织提供一个更合适、更通用的系统。
