Zhang Gui Quan
Guangdong Provincial Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
Yi Chuan. 2019 Aug 20;41(8):754-760. doi: 10.16288/j.yczz.19-105.
Breeding by design is a new concept proposed in the beginning of the century. It refers to the breeding of varieties by crop design utilizing favorable alleles dispersed in different genetic resources in a genome. In the past 20 years, we have proposed a "three-step" strategy to carry out the research on breeding by design in rice. Firstly, we constructed a library of chromosomal single-segment substitution lines (SSSLs) by using of Huajingxian74 (HJX74), an elite xian (indica) variety from South China as the recipient and 43 accessions of seven species of rice AA genome as donors. The genes in the substituted segments of SSSLs were then detected. Breeding by design was conducted by selecting the favorable genes from the SSSL library. Our practice indicates that the SSSL library is a powerful platform for breeding by design and various "traits", "lines" and "varieties" of rice can be designed and bred by utilizing abundant genes in the SSSL library. Here, we introduce the platform of the HJX74-SSSL library and our work of breeding by design on the platform. It will provide a case study for crop design.
设计育种是本世纪初提出的一个新概念。它指的是通过作物设计，利用基因组中分散在不同遗传资源中的有利等位基因来培育品种。在过去20年里，我们提出了一个“三步走”战略来开展水稻设计育种研究。首先，我们以中国南方的一个优良籼稻品种华粳籼74（HJX74）为受体，以水稻AA基因组的7个物种的43份材料为供体，构建了一个染色体单片段代换系（SSSL）文库。然后对SSSLs代换片段中的基因进行检测。通过从SSSL文库中选择有利基因来进行设计育种。我们的实践表明，SSSL文库是设计育种的一个强大平台，利用SSSL文库中的丰富基因，可以设计和培育出水稻的各种“性状”“株系”和“品种”。在此，我们介绍HJX74-SSSL文库平台以及我们在该平台上的设计育种工作。它将为作物设计提供一个案例研究。
