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开发一种新型透明质酸膜治疗眼表疾病。

Development of a novel hyaluronic acid membrane for the treatment of ocular surface diseases.

机构信息

Department of Ophthalmology, Dongguk University, Ilsan Hospital, 814, Siksadong, Ilsan-dong-gu, Goyang, Kyunggido, 410-773, South Korea.

Department of Biochemistry, Dongguk University, College of Medicine, Gyeongju, South Korea.

出版信息

Sci Rep. 2021 Jan 27;11(1):2351. doi: 10.1038/s41598-021-81983-1.

DOI:10.1038/s41598-021-81983-1
PMID:33504908
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7840674/
Abstract

Ocular surface diseases (OSD) can cause serious visual deterioration and discomfort. Commercial artificial tear solution containing hyaluronic acid (HA) show excellent biocompatibility and unique viscoelastic characteristics. Here, we developed a novel HA membrane (HAM) by chemical crosslinking using 1,4-butanediol diglycidyl ether for the effective treatment of OSDs. The main purpose of HAMs is to provide sustained release of HA to modulate the wound healing response in OSDs. The safety and efficacy of HAMs were investigated using primary cultured human corneal epithelial cells and various OSD rabbit models. In the dry state, the HAM is firm, transparent, and easy to manipulate. When hydrated, it swells rapidly with high water retention and over 90% transmission of visible light. Human corneal epithelial cells and rabbit eyes showed no toxic response to HAM. Addition of HAMs to the culture medium enhanced human corneal epithelial cell viability and expression of cell proliferation markers. Investigation of HAM wound healing efficacy using mechanical or chemical corneal trauma and conjunctival surgery in rabbits revealed that application of HAMs to the ocular surface enhanced healing of corneal epithelium and reduced corneal limbal vascularization, opacity and conjunctival fibrosis. The therapeutic potential of HAMs in various OSDs was successfully demonstrated.

摘要

眼表疾病（OSD）可导致严重的视力恶化和不适。含有透明质酸（HA）的商业人工泪液显示出极好的生物相容性和独特的粘弹性特征。在这里，我们使用 1,4-丁二醇二缩水甘油醚通过化学交联开发了一种新型 HA 膜（HAM），用于有效治疗 OSD。HAM 的主要目的是提供 HA 的持续释放，以调节 OSD 中的伤口愈合反应。使用原代培养的人角膜上皮细胞和各种 OSD 兔模型研究了 HAMs 的安全性和功效。在干燥状态下，HAM 坚固、透明且易于操作。水合时，它迅速膨胀，具有高保水能力和超过 90%的可见光透过率。人角膜上皮细胞和兔眼对 HAM 无毒性反应。在培养基中添加 HAMs 可提高人角膜上皮细胞的活力和细胞增殖标志物的表达。使用机械或化学性角膜创伤和结膜手术研究 HAM 伤口愈合功效，发现将 HAMs 应用于眼表可促进角膜上皮愈合，并减少角膜缘血管化、混浊和结膜纤维化。成功证明了 HAMs 在各种 OSD 中的治疗潜力。

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