Schuhegger Regina, Rauhut Thomas, Glawischnig Erich
Lehrstuhl für Genetik, Technische Universität München, Am Hochanger 8, D-85350 Freising, Germany.
J Plant Physiol. 2007 May;164(5):636-44. doi: 10.1016/j.jplph.2006.04.012.
The anthranilate synthase ASA1, CYP79B2 and CYP71B15 (PAD3) are biosynthetic genes of the Arabidopsis phytoalexin camalexin, which are induced after pathogen infection and abiotic treatments like silver nitrate spraying. The natural variation of camalexin biosynthesis in response to Pseudomonas syringae infection was determined in several ecotypes, and differential CYP71B15 regulation as a potential basis for this variation was investigated. The expression of camalexin biosynthetic genes was restricted to the tissue undergoing cell death. After droplet infection with Alternaria alternata, a potent camalexin inducer in the Col-0 ecotype, camalexin formation and the induction of ASA1, CYP79B2 and CYP71B15 were strictly co-localized with the infection site.
邻氨基苯甲酸合酶ASA1、CYP79B2和CYP71B15（PAD3）是拟南芥植物抗毒素camalexin的生物合成基因，在病原体感染和诸如硝酸银喷洒等非生物处理后被诱导。在几种生态型中测定了camalexin生物合成对丁香假单胞菌感染的自然变异，并研究了作为这种变异潜在基础的CYP71B15差异调控。camalexin生物合成基因的表达局限于经历细胞死亡的组织。在用链格孢菌进行液滴感染后，链格孢菌是Col-0生态型中一种强效的camalexin诱导剂，camalexin的形成以及ASA1、CYP79B2和CYP71B15的诱导与感染部位严格共定位。
