Nork T Michael, Kim Charlene B Y, Shanmuganayagam Dhanansayan, Van Lysel Michael S, Ver Hoeve James N, Folts John D
Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison 53792-3220, USA.
Arch Ophthalmol. 2006 Jun;124(6):860-8. doi: 10.1001/archopht.124.6.860.
To develop a quantitative measure of regional variation in choroidal blood flow (ChBF).
Five million 15-microm fluorescent microspheres were injected into the left ventricles of 4 rabbits and 3 monkeys. The fixed globes were bleached, flat mounted, and photomicrographed. After image analysis to locate each microsphere, regional densities and blood flow were determined.
Regional variation in ChBF was clearly evident. In the rabbit, a high density of spheres was seen in the visual streak. This was surrounded by a middle peripheral area of low sphere density and a far peripheral region of moderately high density. In the monkeys, sphere density was markedly greater in the macula compared with the periphery. Contour plots produced lines of constant flow that were oval and extended farther nasally than temporally in the monkeys. The ratio of central to peripheral ChBF was much greater in the monkeys than in the rabbits.
Quantitative assessment of regional ChBF can be performed using a modification of the fluorescent microsphere impaction method.
This method of determining regional ChBF will be useful for studying the vascular effects of pharmacologic agents and for characterizing animal models of human disease involving the outer retina.
开发一种测量脉络膜血流（ChBF）区域差异的定量方法。
将500万个15微米的荧光微球注入4只兔子和3只猴子的左心室。将固定好的眼球进行漂白、平铺并拍照。经过图像分析定位每个微球后，确定区域密度和血流情况。
ChBF的区域差异明显。在兔子中，视条纹处可见高密度的微球。其周围是微球密度低的中周区域和密度适中高的远周区域。在猴子中，黄斑处的微球密度明显高于周边区域。等高线图显示，猴子的恒定血流线呈椭圆形，向鼻侧延伸的距离比颞侧更远。猴子中央与周边ChBF的比值比兔子大得多。
使用荧光微球撞击法的改进方法可对ChBF区域进行定量评估。
这种确定ChBF区域的方法将有助于研究药物的血管效应，并用于表征涉及外视网膜的人类疾病动物模型。
