Crompton K E, Tomas D, Finkelstein D I, Marr M, Forsythe J S, Horne M K
Department of Materials Engineering, Monash University, Wellington Road, Clayton, 3168, Australia.
J Mater Sci Mater Med. 2006 Jul;17(7):633-9. doi: 10.1007/s10856-006-9226-6.
Chitosan is a well-known biomaterial that, with the addition of glycerophosphate salt (GP), gels at physiological temperatures and therefore is useful for tissue engineering purposes. This study examines the procedure of injecting chitosan/ GP to the brain in order to form a gel track. The gel system and surgical technique were successful in this endeavour; however, on examining the inflammatory response to the material it was found that the chitosan/GP was wholly engulfed by macrophages within 7 days. This was determined by staining for both the gel and the macrophages, an important technique for localising injected material. The chitosan/GP-containing macrophages formed a neat tract at the lesion site, but after 45 days no chitosan/GP was found. It was concluded that, although chitosan/GP is present after implantation, it is not available for direct scaffolding in the brain.
壳聚糖是一种著名的生物材料，添加甘油磷酸盐（GP）后，它在生理温度下会形成凝胶，因此可用于组织工程目的。本研究考察了向大脑注射壳聚糖/GP以形成凝胶轨迹的过程。在这一尝试中，凝胶系统和手术技术取得了成功；然而，在检查对该材料的炎症反应时发现，壳聚糖/GP在7天内被巨噬细胞完全吞噬。这是通过对凝胶和巨噬细胞进行染色来确定的，这是定位注射材料的一项重要技术。含壳聚糖/GP的巨噬细胞在损伤部位形成了一条整齐的轨迹，但45天后未发现壳聚糖/GP。得出的结论是，尽管壳聚糖/GP在植入后存在，但它无法在大脑中直接用作支架。
