Baxter R L, Baxter H C, Campbell G A, Grant K, Jones A, Richardson P, Whittaker G
School of Chemistry, University of Edinburgh, Edinburgh, UK.
J Hosp Infect. 2006 Aug;63(4):439-44. doi: 10.1016/j.jhin.2006.03.011. Epub 2006 Jun 12.
'Ready-for-use' instruments from surgical instrument trays were examined after routine cleaning and sterilization in a blinded study. These reprocessed instruments originated from five National Health Service hospital trust sterile service departments in England and Wales. Determination of residual protein and peptide contamination was carried out by acid stripping of the instrument surfaces, hydrolysis of the constituent amino acids and quantitative total amino acid analysis. One hundred and twenty instruments were analysed, and the median levels of residual protein contamination per instrument for the individual trays were 267, 260, 163, 456 and 756 microg. Scanning electron microscopy and energy dispersive X-ray spectroscopic analyses of the instruments showed that tissue deposits were localized on surfaces, but there was no significant correlation between overall protein soiling and instrument complexity. The highest levels of residual contamination were found on instruments used for tonsillectomy and adenoid surgery.
在一项盲法研究中，对手术器械托盘上经常规清洁和灭菌后的“即用型”器械进行了检查。这些再处理后的器械来自英格兰和威尔士的五个国民保健服务医院信托无菌服务部门。通过对器械表面进行酸剥离、对组成氨基酸进行水解以及进行定量总氨基酸分析来测定残留蛋白质和肽污染情况。共分析了120件器械，各个托盘每件器械的残留蛋白质污染中位数水平分别为267、260、163、456和756微克。对器械进行扫描电子显微镜和能量色散X射线光谱分析表明，组织沉积物位于表面，但总体蛋白质污染与器械复杂程度之间无显著相关性。扁桃体切除术和腺样体手术所用器械的残留污染水平最高。
