Bruna Camila Quartim de Moraes, Souza Rafael Queiroz de, Massaia Irineu Francisco Silva, Cruz Áurea Silveira, Graziano Kazuko Uchikawa
PhD, Visiting Professor, Faculdade de Ciências Médicas, Santa Casa de São Paulo, São Paulo, SP, Brazil.
PhD, Researcher, Escola de Enfermagem, Universidade de São Paulo, São Paulo, SP, Brazil.
Rev Lat Am Enfermagem. 2016 Oct 10;24:e2805. doi: 10.1590/1518-8345.1127.2805.
to determine if there are differences on the safety of the preparation of clean surgical instruments using different types of gloves and bare hands and evaluate the microbiological load of these preparations without gloves.
laboratory procedure with a pragmatic approach, in which the samples were handled with different types of gloves and bare hands. In addition, cytotoxicity assays were carried out by means of the agar diffusion method. Further samples were subjected to microbiological analysis after being handled without gloves.
none of the samples showed cytotoxic effect. All microbiological cultures showed growth of microorganisms, but no microorganism has been recovered after autoclaving.
there were no differences in the cytotoxic responses regarding the use of different types of gloves and bare hands in the handling of clean surgical instruments, which could entail iatrogenic risk. It is noteworthy that the use of gloves involves increase in the costs of process and waste generation, and the potential allergenic risk to latex.
确定使用不同类型手套和徒手准备清洁手术器械在安全性上是否存在差异，并评估无手套操作时这些准备工作的微生物负荷。
采用实用方法的实验室程序，其中样本用不同类型手套和徒手处理。此外，通过琼脂扩散法进行细胞毒性试验。进一步的样本在无手套操作后进行微生物分析。
所有样本均未显示细胞毒性作用。所有微生物培养均显示有微生物生长，但高压灭菌后未检出任何微生物。
在处理清洁手术器械时，使用不同类型手套和徒手操作在细胞毒性反应方面没有差异，这可能带来医源性风险。值得注意的是，使用手套会增加过程成本和废物产生，以及对乳胶的潜在过敏风险。
