Pathology Department, Stavanger University Hospital, Armauer Hansen Road 20, PO Box 8100, 4068 Stavanger, Norway.
Cell Oncol (Dordr). 2011 Oct;34(5):443-50. doi: 10.1007/s13402-011-0047-3. Epub 2011 May 15.
Cervical intraepithelial neoplasia (CIN), a frequently encountered disease caused by Human Papilloma Virus (HPV) is often diagnosed in formaldehyde-fixed paraffin embedded (FFPE) punch biopsies. Since it is known that this procedure strongly affects the water-soluble proteins contained in the cervical tissue we decided to investigate whether a water-soluble protein-saving biopsy processing method can be used to support the diagnosis of normal and CIN.
Cervical punch biopsies from 55 women were incubated for 24 h at 4°C in RPMI1640 medium for protein analysis prior to usual FFPE processing and p16 and Ki67-supported histologic consensus diagnosis was assessed. The biopsy supernatants were subjected to surface-enhanced laser desorption-ionization time of flight mass spectrometry (SELDI-TOF MS) for identifying differentially expressed proteins. Binary logistic regression and classification and regression trees (CART) were used to develop a classification model.
The age of the patients ranged from 26 to 40 years (median 29.7). The consensus diagnoses were normal cervical tissue (n = 10) and CIN2-3 (n = 45). The mean protein concentration was 1.00 and 1.09 mg/ml in the normal and CIN2-3 group, respectively. The peak detection and clustering process resulted in 40 protein peaks. Many of these peaks differed between the two groups, but only three had independent discriminating power. The overall classification results were 88%.
Water-soluble proteins sampled from punch biopsies are promising to assist the diagnosis of normal and CIN2-3.
宫颈上皮内瘤变（CIN）是一种由人乳头瘤病毒（HPV）引起的常见疾病，通常在福尔马林固定石蜡包埋（FFPE）穿刺活检中诊断。由于众所周知，这种程序会强烈影响宫颈组织中含有的水溶性蛋白质，因此我们决定研究是否可以使用水溶性蛋白质保存活检处理方法来支持正常和 CIN 的诊断。
将 55 名女性的宫颈穿刺活检在 RPMI1640 培养基中于 4°C 孵育 24 小时，用于蛋白质分析，然后进行常规 FFPE 处理，并评估 p16 和 Ki67 支持的组织学共识诊断。将活检上清液进行表面增强激光解吸电离飞行时间质谱（SELDI-TOF MS）分析，以鉴定差异表达的蛋白质。使用二元逻辑回归和分类回归树（CART）建立分类模型。
患者年龄为 26 至 40 岁（中位数 29.7 岁）。共识诊断为正常宫颈组织（n=10）和 CIN2-3（n=45）。正常和 CIN2-3 组的平均蛋白质浓度分别为 1.00 和 1.09mg/ml。峰检测和聚类过程产生了 40 个蛋白质峰。这两组之间的许多峰都不同，但只有三个具有独立的鉴别能力。总体分类结果为 88%。
从穿刺活检中提取的水溶性蛋白质有希望辅助诊断正常和 CIN2-3。
