Al-Younes Hesham M, Gussmann Joscha, Braun Peter R, Brinkmann Volker, Meyer Thomas F
J Med Microbiol. 2006 Jul;55(Pt 7):879-886. doi: 10.1099/jmm.0.46445-0.
The differential influence of individual amino acids on the growth of Chlamydia trachomatis versus Chlamydia (Chlamydophila) pneumoniae was investigated. Certain essential amino acids added in excess at the middle of the infection course resulted in varying degrees of abnormality in the development of the two species. If amino acids were added as early as 2 h post-infection, these effects were even more pronounced. The most effective amino acids in terms of C. trachomatis growth inhibition were leucine, isoleucine, methionine and phenylalanine. These amino acids elicited similar effects against C. pneumoniae, except methionine, which, surprisingly, showed a lower inhibitory activity. Tryptophan and valine marginally inhibited C. trachomatis growth and, paradoxically, led to a considerable enhancement of C. pneumoniae growth. On the other hand, some non-essential amino acids administered at the middle of or throughout the infection course differentially affected the development of the two species. For example, C. trachomatis growth was efficiently inhibited by glycine and serine, whereas C. pneumoniae was relatively less sensitive to these agents. Another difference was apparent for glutamate, glutamine and aspartate, which stimulated C. pneumoniae growth more than that of C. trachomatis. Overall, several distinctive patterns of susceptibility to excess amino acid levels were revealed for two representative C. trachomatis and C. pneumoniae isolates. Perturbation of amino acid levels, e.g. of leucine and isoleucine, might form a basis for the development of novel treatment or preventive regimens for chlamydial diseases.
研究了单个氨基酸对沙眼衣原体与肺炎衣原体（嗜衣原体属）生长的不同影响。在感染过程中期过量添加某些必需氨基酸会导致这两种衣原体的发育出现不同程度的异常。如果在感染后2小时尽早添加氨基酸，这些影响会更加明显。就抑制沙眼衣原体生长而言，最有效的氨基酸是亮氨酸、异亮氨酸、蛋氨酸和苯丙氨酸。这些氨基酸对肺炎衣原体也有类似作用，但令人惊讶的是，蛋氨酸的抑制活性较低。色氨酸和缬氨酸对沙眼衣原体生长的抑制作用微弱，相反，却能显著促进肺炎衣原体的生长。另一方面，在感染过程中期或整个过程中添加的一些非必需氨基酸对这两种衣原体的发育有不同影响。例如，甘氨酸和丝氨酸能有效抑制沙眼衣原体生长，而肺炎衣原体对这些物质相对不敏感。谷氨酸、谷氨酰胺和天冬氨酸也有明显差异，它们对肺炎衣原体生长的刺激作用比对沙眼衣原体的更强。总体而言，揭示了两种代表性沙眼衣原体和肺炎衣原体分离株对过量氨基酸水平的几种独特敏感模式。氨基酸水平的扰动，如亮氨酸和异亮氨酸的扰动，可能为开发针对衣原体疾病的新治疗或预防方案奠定基础。
