痘病毒CD8 + T细胞决定簇及BALB/c小鼠中的交叉反应性
Poxvirus CD8+ T-cell determinants and cross-reactivity in BALB/c mice.
作者信息
Tscharke David C, Woo Wai-Ping, Sakala Isaac G, Sidney John, Sette Alessandro, Moss Denis J, Bennink Jack R, Karupiah Gunasegaran, Yewdell Jonathan W
机构信息
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases/NIH, Bethesda, MD 20892, USA.
出版信息
J Virol. 2006 Jul;80(13):6318-23. doi: 10.1128/JVI.00427-06.
Abstract
Mouse models of orthopoxvirus disease provide great promise for probing basic questions regarding host responses to this group of pathogens, which includes the causative agents of monkeypox and smallpox. However, some essential tools for their study that are taken for granted with other mouse models are not available for these viruses. Here we map and characterize the initial CD8+ T-cell determinants for poxviruses in H-2d-haplotype mice. CD8+ T cells recognizing these three determinants make up around 40% of the total responses to vaccinia virus during and after resolution of infection. We then use these determinants to test if predicted conservation across orthopoxvirus species matches experimental observation and find an unexpectedly cross-reactive variant peptide encoded by ectromelia (mousepox) virus.
摘要
正痘病毒病的小鼠模型为探究宿主对这组病原体（包括猴痘和天花的病原体）的反应的基本问题提供了巨大希望。然而，对于这些病毒，一些在其他小鼠模型中被视为理所当然的研究必需工具却无法获得。在这里，我们绘制并表征了H-2d单倍型小鼠中正痘病毒的初始CD8 + T细胞决定簇。识别这三个决定簇的CD8 + T细胞在感染消退期间及之后对痘苗病毒的总反应中约占40%。然后，我们使用这些决定簇来测试正痘病毒物种间预测的保守性是否与实验观察结果相符，并发现了由埃可病毒（鼠痘）编码的一个意外的交叉反应性变异肽。
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