Espinoza C, Vega A, Medina C, Schlauch K, Cramer G, Arce-Johnson P
Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla 114-D, Alameda 340, Santiago, Chile.
Funct Integr Genomics. 2007 Apr;7(2):95-110. doi: 10.1007/s10142-006-0031-6. Epub 2006 Jun 15.
Viral diseases affect grapevine cultures without inducing any resistance response. Thus, these plants develop systemic diseases and are chronically infected. Molecular events associated with viral compatible infections responsible for disease establishment and symptoms development are poorly understood. In this study, we surveyed viral infection in grapevines at a transcriptional level. Gene expression in the Vitis vinifera red wine cultivars Carménère and Cabernet-Sauvignon naturally infected with GLRaV-3 were evaluated using a genome-wide expression profiling with the Vitis vinifera GeneChip from Affymetrix. We describe numerous genes that are induced or repressed in viral infected grapevines leaves. Changes in gene expression involved a wide spectrum of biological functions, including processes of translation and protein targeting, metabolism, transport, and cell defense. Considering cellular localization, the membrane and endomembrane systems appeared with the highest number of induced genes, while chloroplastic genes were mostly repressed. As most induced genes associated with the membranous system are involved in transport, the possible effect of virus in this process is discussed. Responses of both cultivars are analyzed and the results are compared with published data from other species. This is the first study of global gene profiling in grapevine in response to viral infections using DNA microarray.
病毒病害侵袭葡萄栽培品种时不会引发任何抗性反应。因此，这些植株会患上系统性疾病并受到慢性感染。与导致疾病发生和症状发展的病毒相容性感染相关的分子事件，目前了解甚少。在本研究中，我们在转录水平上对葡萄中的病毒感染情况进行了调查。利用Affymetrix公司的葡萄基因芯片进行全基因组表达谱分析，评估了自然感染葡萄卷叶相关病毒3型（GLRaV-3）的酿酒葡萄品种佳美娜和赤霞珠中的基因表达。我们描述了许多在病毒感染的葡萄叶片中被诱导或抑制的基因。基因表达的变化涉及广泛的生物学功能，包括翻译和蛋白质靶向、代谢、运输以及细胞防御等过程。考虑到细胞定位，膜和内膜系统中出现诱导基因的数量最多，而叶绿体基因大多被抑制。由于大多数与膜系统相关的诱导基因都参与运输，因此讨论了病毒在此过程中可能产生的影响。分析了两个品种的反应，并将结果与其他物种已发表的数据进行了比较。这是首次利用DNA微阵列对葡萄响应病毒感染进行全基因组基因谱分析的研究。
