Rubio Manuel, Martínez-García Pedro J, Nikbakht-Dehkordi Azam, Prudencio Ángela S, Gómez Eva M, Rodamilans Bernardo, Dicenta Federico, García Juan A, Martínez-Gómez Pedro
Department of Plant Breeding, CEBAS-CSIC, P.O. Box 164, 30100 Murcia, Spain.
Department of Plant Breeding and Biotechnology, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari, Iran.
Int J Mol Sci. 2021 Mar 30;22(7):3585. doi: 10.3390/ijms22073585.
No natural sources of resistance to (PPV, sharka disease) have been identified in peach. However, previous studies have demonstrated that grafting a "Garrigues" almond scion onto "GF305" peach rootstock seedlings heavily infected with PPV can progressively reduce disease symptoms and virus accumulation. Furthermore, grafting a "Garrigues" scion onto the "GF305" rootstock has been shown to completely prevent virus infection. This study aims to analyse the rewiring of gene expression associated with this resistance to PPV transmitted by grafting through the phloem using RNA-Seq and RT-qPCR analysis. A total of 18 candidate genes were differentially expressed after grafting "Garrigues" almond onto healthy "GF305" peach. Among the up-regulated genes, a homolog stands out, which, together with the differential expression of - and -homologs, suggests that the RNA silencing machinery is activated by PPV infection and can contribute to the resistance induced by "Garrigues" almond. could be also relevant for the "Garrigues"-induced response, since its expression is much higher in "Garrigues" than in "GF305". We also discuss the potential relevance of the following in PPV infection and "Garrigues"-induced resistance: several pathogenesis-related proteins; no apical meristem proteins; the transcription initiation factor, TFIIB; the speckle-type POZ protein; in addition to a number of proteins involved in phytohormone signalling.
在桃中尚未发现对李痘病毒（PPV，即卷叶病）的天然抗性来源。然而，先前的研究表明，将“加里格斯”扁桃接穗嫁接到严重感染PPV的“GF305”桃砧木幼苗上，可逐渐减轻病害症状并减少病毒积累。此外，将“加里格斯”接穗嫁接到“GF305”砧木上已被证明可完全防止病毒感染。本研究旨在通过RNA测序和逆转录定量PCR分析，分析与通过韧皮部嫁接传递的对PPV抗性相关的基因表达重编程。将“加里格斯”扁桃嫁接到健康的“GF305”桃上后，共有18个候选基因差异表达。在上调的基因中，一个同源物尤为突出，它与同源物和同源物的差异表达一起，表明RNA沉默机制被PPV感染激活，并可能有助于“加里格斯”扁桃诱导的抗性。也可能与“加里格斯”诱导的反应相关，因为它在“加里格斯”中的表达远高于在“GF305”中的表达。我们还讨论了以下因素在PPV感染和“加里格斯”诱导的抗性中的潜在相关性：几种病程相关蛋白；无顶端分生组织蛋白；转录起始因子TFIIB；斑点型POZ蛋白；此外还有一些参与植物激素信号传导的蛋白。
