González-López Claudia Isela, Ortiz-Castellanos Lucila, Ruiz-Herrera José
Departamento de Ingeniería Genética, Unidad de Biotecnología e Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Gto., México.
Curr Microbiol. 2006 Jul;53(1):8-12. doi: 10.1007/s00284-005-0070-6. Epub 2006 May 29.
Yarrowia lipolytica is a dimorphic fungus that secretes either an acidic or an alkaline protease depending on the environmental pH. Previous results have indicated that secretion of the alkaline protease is under control of the pH signaling Pal/Rim pathway originally described in Aspergillus nidulans. Several Y. lipolytica mutants defective in some Rim components of this pathway have been previously isolated and the RIM genes characterized. In the present study, Y. lipolytica RIM9 (palI) gene (YlRIM9) was sequenced from a plasmid (AL414126) of the Genolevures project (the DNA sequence data for YlRIM9 gene has been deposited at EMBL with accession number AJ566902). The derived translation product contains 724 amino acids with a predicted signal peptide and four transmembrane domains in its N-terminal region. We demonstrated that mutation in YlRIM9, as well as in other genes encoding members of the Pal/Rim pathway, did not affect the pH-dependent dimorphic transition of Y. lipolytica. A different pathway must exist in this fungus that controls the effect of pH on dimorphism.
解脂耶氏酵母是一种二态真菌，根据环境pH值分泌酸性或碱性蛋白酶。先前的结果表明，碱性蛋白酶的分泌受最初在构巢曲霉中描述的pH信号Pal/Rim途径的控制。此前已分离出该途径某些Rim成分存在缺陷的几种解脂耶氏酵母突变体，并对RIM基因进行了表征。在本研究中，从Genolevures项目的一个质粒（AL414126）中对解脂耶氏酵母RIM9（palI）基因（YlRIM9）进行了测序（YlRIM9基因的DNA序列数据已保存在EMBL，登录号为AJ566902）。推导的翻译产物包含724个氨基酸，在其N端区域有一个预测的信号肽和四个跨膜结构域。我们证明，YlRIM9以及编码Pal/Rim途径成员的其他基因中的突变不会影响解脂耶氏酵母的pH依赖性二态转变。这种真菌中一定存在不同的途径来控制pH对二态性的影响。
