Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 629, CP. 36500, Irapuato, Gto, Mexico.
Res Microbiol. 2012 Jun;163(5):378-87. doi: 10.1016/j.resmic.2012.03.002. Epub 2012 May 14.
Fungal dimorphism is the capacity of certain species of fungi to grow in the form of budding yeasts or mycelium depending on the environmental conditions. This characteristic is a complex phenomenon that involves modifications of the molecular machinery in response to different environmental signals. Through the use of microarrays, in this work we identified genes involved in the early stages of the yeast-to-mycelium transition of Yarrowia lipolytica induced by a shift in pH of the medium. As controls, yeast and mycelium monomorphic mutants were used, identifying by this mean a total of 61 upregulated and 165 downregulated genes specifically involved in dimorphism. Determination of the putative function of these genes was accomplished by means of BLAST analyses which showed that they were involved mainly in processes such as remodeling and biogenesis of the cell wall, membrane trafficking and N- or O-glycosylation. Some of these genes were identified by homology with Saccharomyces cerevisiae genes, and found to play a role during the dimorphic transition in both systems.
真菌的二态性是指某些真菌物种能够根据环境条件生长为出芽酵母或菌丝体。这种特征是一种复杂的现象，涉及到分子机制的改变以响应不同的环境信号。在这项工作中，我们使用微阵列技术鉴定了与 Yarrowia lipolytica 中由介质 pH 变化引起的酵母到菌丝体转变的早期阶段相关的基因。作为对照，使用了酵母和菌丝体单态突变体，通过这种方法总共鉴定出 61 个上调和 165 个下调基因，这些基因专门参与二态性。通过 BLAST 分析确定这些基因的假定功能表明，它们主要参与细胞壁的重塑和生物发生、膜运输和 N 或 O-糖基化等过程。其中一些基因与酿酒酵母基因具有同源性，并在两个系统的二态性转变过程中发挥作用。
