Albera C, Mabritto I, Ghio P, Scagliotti G V, Pozzi E
Clinical and Biological Sciences Department, University of Turin, Italy.
Respiration. 1991;58(2):65-71. doi: 10.1159/000195899.
Lymphocyte subpopulations analysis by an 11-monoclonal antibody (MoAb) panel was carried out in pleural fluid and in peripheral blood in 30 patients affected by newly diagnosed, untreated pleural effusion of different etiology determinated with bacteriological, cytological or histological criteria. Lymphocytes were the predominant cell type, in pleural fluid, in neoplastic pleural effusions as well as in congestive heart failure pleural effusions and, especially, in tuberculous pleural effusions. Lymphocyte analysis in pleural fluid and in peripheral blood suggests the involvement of different mechanisms for the lymphocyte accumulation in the pleural space according to different etiologies. Tuberculous pleural effusions showed an evident CD4+ and TEC T5.9+ lymphocyte accumulation from peripheral blood. In these patients, cutaneous skin test response to purified protein derivative was strongly related to this situation. In neoplastic pleural effusions there was a lower percentage of CD4+ lymphocytes, reflecting circulating lymphocyte pool; however, in neoplastic pleural effusions, various lymphocyte patterns may be sometimes observed depending on different histologies. Passive lymphocyte accumulation seems to be the most important mechanism in congestive-heart-failure pleural effusions.
采用11种单克隆抗体（MoAb）组合对30例新诊断的、未经治疗的不同病因胸腔积液患者的胸腔积液和外周血进行淋巴细胞亚群分析，病因通过细菌学、细胞学或组织学标准确定。淋巴细胞是胸腔积液中的主要细胞类型，在肿瘤性胸腔积液、充血性心力衰竭胸腔积液中，尤其是在结核性胸腔积液中。胸腔积液和外周血中的淋巴细胞分析表明，根据不同病因，胸腔内淋巴细胞积聚涉及不同机制。结核性胸腔积液显示外周血中明显的CD4 +和TEC T5.9 +淋巴细胞积聚。在这些患者中，对纯化蛋白衍生物的皮肤试验反应与这种情况密切相关。肿瘤性胸腔积液中CD4 +淋巴细胞的百分比更低，反映了循环淋巴细胞池；然而，在肿瘤性胸腔积液中，根据不同组织学有时可能观察到各种淋巴细胞模式。被动淋巴细胞积聚似乎是充血性心力衰竭胸腔积液中最重要的机制。
