Aguiar Lucia Maria Zanatta de, Antonangelo Leila, Vargas Francisco S, Zerbini Maria Cláudia Nogueira, Sales Maria Mirtes, Uip David E, Saldiva Paulo Hilário Nascimento
Pulmonary Division, Heart Institute, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil.
Clinics (Sao Paulo). 2008 Oct;63(5):637-44. doi: 10.1590/s1807-59322008000500012.
Tuberculosis and cancer are the main causes of pleural effusion. Pleural involvement is associated with migration of immune cells to the pleural cavity. We sought to characterize the immunophenotype of leukocytes in the pleural effusion and peripheral blood of patients with tuberculosis or malignancy.
Thirty patients with tuberculosis (14) or malignancy (16) were studied. A control group included 20 healthy blood donors.
Malignant phycoerythrin pleural effusions showed higher percentages of CD3, CD4, CD3CD45RO, and CD20CD25 lymphocytes and lower percentages of CD3CD25 and CD20HLA-DR when compared to PB lymphocytes. Compared to PB, tuberculous effusions had a higher percentage of lymphocytes that co-expressed CD3, CD4, CD3CD45RO, CD3TCRalphabeta, CD3CD28, and CD20 and a lower percentage of CD14, CD8 and CD3TCRgammadelta-positive lymphocytes. Malignant effusions presented higher expression of CD14 whereas tuberculous effusions had higher expression of CD3 and CD3CD95L. Peripheral blood cells from tuberculosis patients showed higher expression of CD14, CD20CD25 and CD3CD95L. Compared with the control cells, tuberculosis and cancer peripheral blood cells presented a lower percentage of CD3CD4 and CD3CD28-positive cells as well as a higher percentage of CD3CD8, CD3CD25 and CD3CD80-positive cells.
Tuberculous and malignant peripheral blood is enriched with lymphocytes with a helper/inducer T cell phenotype, which are mainly of memory cells. CD14-positive cells were more frequently found in malignant effusions, while CD3-positive cells expressing Fas ligand were more frequently found in tuberculous effusions.
结核病和癌症是胸腔积液的主要病因。胸膜受累与免疫细胞向胸腔迁移有关。我们试图对结核病或恶性肿瘤患者胸腔积液和外周血中白细胞的免疫表型进行特征描述。
对30例结核病患者（14例）或恶性肿瘤患者（16例）进行了研究。一个对照组包括20名健康献血者。
与外周血淋巴细胞相比，恶性藻红蛋白胸腔积液中CD3、CD4、CD3CD45RO和CD20CD25淋巴细胞的百分比更高，而CD3CD25和CD20HLA-DR的百分比更低。与外周血相比，结核性胸腔积液中共同表达CD3、CD4、CD3CD45RO、CD3TCRαβ、CD3CD28和CD20的淋巴细胞百分比更高，而CD14、CD8和CD3TCRγδ阳性淋巴细胞的百分比更低。恶性胸腔积液中CD14表达较高，而结核性胸腔积液中CD3和CD3CD95L表达较高。结核病患者的外周血细胞中CD14、CD20CD25和CD3CD95L表达较高。与对照细胞相比，结核病和癌症外周血细胞中CD3CD4和CD3CD28阳性细胞的百分比更低，而CD3CD8、CD3CD25和CD3CD80阳性细胞的百分比更高。
结核病和恶性肿瘤外周血中富含具有辅助/诱导性T细胞表型的淋巴细胞，主要是记忆细胞。CD14阳性细胞在恶性胸腔积液中更常见，而表达Fas配体的CD3阳性细胞在结核性胸腔积液中更常见。
