Lamprianou Smaragda, Vacaresse Nathalie, Suzuki Yoshihisa, Meziane Hamid, Buxbaum Joseph D, Schlessinger Joseph, Harroch Sheila
Institut Pasteur, Department of Neuroscience, 25 Rue du Dr. Roux, 75724 Paris, France.
Mol Cell Biol. 2006 Jul;26(13):5106-19. doi: 10.1128/MCB.00101-06.
In order to gain insight into the biological role of receptor protein tyrosine phosphatase gamma (RPTPgamma), we have generated RPTPgamma-null mice. RPTPgamma was disrupted by insertion of the beta-galactosidase gene under the control of the RPTPgamma promoter. As the RPTPgamma-null mice did not exhibit any obvious phenotype, we made use of these mice to study RPTPgamma expression and thus shed light on potential biological functions of this phosphatase. Inspection of mouse embryos shows that RPTPgamma is expressed in a variety of tissues during embryogenesis. RPTPgamma is expressed in both embryonic and adult brains. Specifically, we detected RPTPgamma expression in cortical layers II and V and in the stratum pyramidale of the hippocampus, indicating that RPTPgamma is a marker for pyramidal neurons. Mixed primary culture of glial cells showed a lack of expression of RPTPgamma in astrocytes and a low expression of RPTPgamma in oligodendrocytes and in microglia. Interestingly, RPTPgamma expression was detected in all sensory organs, including the ear, nose, tongue, eye, and vibrissa follicles, suggesting a potential role of RPTPgamma in sensory neurons. An initial behavioral analysis showed minor changes in the RPTPgamma-null mice.
为了深入了解受体蛋白酪氨酸磷酸酶γ（RPTPγ）的生物学作用，我们培育出了RPTPγ基因敲除小鼠。通过在RPTPγ启动子的控制下插入β-半乳糖苷酶基因，使RPTPγ基因失活。由于RPTPγ基因敲除小鼠未表现出任何明显的表型，我们利用这些小鼠来研究RPTPγ的表达，从而揭示这种磷酸酶的潜在生物学功能。对小鼠胚胎的检查表明，RPTPγ在胚胎发育过程中在多种组织中表达。RPTPγ在胚胎和成年大脑中均有表达。具体而言，我们在皮质层II和V以及海马体的锥体层中检测到了RPTPγ的表达，这表明RPTPγ是锥体神经元的标志物。胶质细胞的原代混合培养显示，星形胶质细胞中缺乏RPTPγ的表达，少突胶质细胞和小胶质细胞中RPTPγ的表达较低。有趣的是，在所有感觉器官中都检测到了RPTPγ的表达，包括耳朵、鼻子、舌头、眼睛和触须毛囊，这表明RPTPγ在感觉神经元中可能发挥作用。初步的行为分析显示，RPTPγ基因敲除小鼠有轻微变化。
