Pagniez G, Radice M, Cuirolo A, Rodríguez O, Rodríguez H, Vay C, Famiglietti A, Gutkind G
Carrera de Especialización en Bioquímica Clínica, área Bacteriología Clínica, Escuela de graduados.
Rev Argent Microbiol. 2006 Jan-Mar;38(1):33-7.
The present study was conducted to estimate the prevalence of metallo-beta-lactamases in 91 consecutive carbapenem resistant Pseudomonas aeruginosa isolates, recovered from inpatients at Hospital de Clínicas in Buenos Aires. Both, phenotypic and genotypic methods detected the presence of carbapenemases in 10 (11%) isolates, corresponding to VIM-11 in 7/10 and VIM-2 in the others. Codifying genes were all included in class 1 integrons, upstream genes coding for aminoglycoside modifying enzymes. One hundred percent sensitivity and specificity was achieved by the metallo-beta-lactamases phenotypic screening method using EDTA (1 micromol) disks in the Pseudomonas aeruginosa isolates included in this study. Sensitivity to aztreonam in carbapenem resistant isolates was suspicious of the presence of these enzymes.
本研究旨在评估从布宜诺斯艾利斯临床医院住院患者中分离出的91株连续的耐碳青霉烯类铜绿假单胞菌中金属β-内酰胺酶的流行情况。表型和基因型方法均在10株（11%）分离株中检测到碳青霉烯酶的存在，其中7/10为VIM-11，其余为VIM-2。编码基因均包含在1类整合子中，其上游基因编码氨基糖苷修饰酶。在本研究纳入的铜绿假单胞菌分离株中，使用EDTA（1微摩尔）纸片的金属β-内酰胺酶表型筛选方法具有100%的敏感性和特异性。耐碳青霉烯类分离株对氨曲南的敏感性可疑存在这些酶。
