通过酶联免疫吸附测定（ELISA）检测蜡样芽孢杆菌鞭毛抗原。

Detection of Bacillus cereus flagellar antigen by enzyme-linked immunosorbent assay (ELISA).

作者信息

Murakami T, Hiraoka K, Mikami T, Matsumoto T, Katagiri S, Suzuki M

机构信息

Department of Microbiology, Tohoku College of Pharmacy, Sendai.

出版信息

Microbiol Immunol. 1991;35(3):223-34. doi: 10.1111/j.1348-0421.1991.tb01551.x.

DOI:10.1111/j.1348-0421.1991.tb01551.x

PMID:1678490

Abstract

A serological typing scheme of Bacillus cereus has been developed by immunochemical analyses of flagellar antigen using an agglutination method. Enzyme-linked immunosorbent assay (ELISA) for the classification of flagellar serotype of Bacillus cereus had greater sensitivity. 10-500 times, than that of agglutination method. The specificity of flagellar antigen and antibody was determined by immunogold electron microscopy and ELISA inhibition assay. Application of ELISA is useful for the detection of the small amounts and many kinds of antigen-antibody reactions.

摘要

通过使用凝集法对鞭毛抗原进行免疫化学分析，已开发出蜡样芽孢杆菌的血清学分型方案。用于蜡样芽孢杆菌鞭毛血清型分类的酶联免疫吸附测定（ELISA）的灵敏度比凝集法高10至500倍。通过免疫金电子显微镜和ELISA抑制试验确定了鞭毛抗原和抗体的特异性。ELISA的应用有助于检测少量和多种抗原-抗体反应。