Montie T C, Anderson T R
Department of Microbiology, University of Tennessee, Knoxville 37996-0845.
Eur J Clin Microbiol Infect Dis. 1988 Apr;7(2):256-60. doi: 10.1007/BF01963097.
An enzyme-linked immunosorbent assay (ELISA) with goat anti-rabbit IgG conjugated to peroxidase was used to test for the two antigen types of Pseudomonas aeruginosa: b (homogeneous) and a (heterogeneous) which contains the common subantigen (a0) and combinations of subtypes (a, a2 a3 a4). Preparations of b-type flagellar antigen could be distinguished from a-type by using b-adsorbed antisera titers as reciprocals of endpoint dilutions exceeding one million. Extracts from nonflagellated bacteria or purified lipopolysaccharides from the same strain were used as controls, which showed only background activity. Unknown flagellar antigen was determined using both isolated antigen preparations and formalin-killed bacterial cells. The ELISA procedure proved much more sensitive than the slide agglutination procedure: whereas nine of 18 strains tested did not react in the slide agglutination procedure all 18 strains were definitively typed as a or b strains with the ELISA. The ELISA also revealed the presence of a dominant, cross-reacting epitope (a0) in the heterogeneous a-type flagella using either isolated antigen or intact cells.
采用与过氧化物酶偶联的山羊抗兔IgG的酶联免疫吸附测定（ELISA）来检测铜绿假单胞菌的两种抗原类型：b（同质）型和a（异质）型，a型包含共同的亚抗原（a0）以及亚型组合（a、a2、a3、a4）。通过使用b吸附抗血清效价（作为终点稀释倍数的倒数，超过一百万），可以将b型鞭毛抗原制剂与a型区分开来。来自无鞭毛细菌的提取物或同一菌株的纯化脂多糖用作对照，仅显示背景活性。使用分离的抗原制剂和福尔马林灭活的细菌细胞来确定未知的鞭毛抗原。ELISA方法比玻片凝集法灵敏得多：在玻片凝集法中，18株测试菌株中有9株无反应，而使用ELISA法可将所有18株菌株明确鉴定为a型或b型菌株。ELISA还揭示，使用分离抗原或完整细胞时，异质a型鞭毛中存在一个显性的交叉反应表位（a0）。
