Mikami T, Hiraoka K, Murakami T, Boon-Long J, Matsumoto T, Suzuki M
Department of Microbiology, Tohoku College of Pharmacy, Miyagi.
Microbiol Immunol. 1990;34(8):709-14. doi: 10.1111/j.1348-0421.1990.tb01048.x.
Bacillus cereus has been classified into 23 types by immunochemical analyses of flagella antigen, but a common antigenic determinant of flagella had not been determined. When the immunochemical method of classification had changed from "agglutination method" to "enzyme-linked immunosorbent assay (ELISA)," the cross-reactivity between each flagella type was increased. These results indicated that the application of ELISA method would enable detection of the common antigenic determinant of B. cereus. Therefore, we attempted to make monoclonal antibody against common flagella antigen that could be detected by ELISA. Monoclonal antibody provided was specific for B. cereus (H-1 to H-23) and did not show the cross-reactivity with Escherichia coli NIH and Bacillus subtilis.
通过对鞭毛抗原进行免疫化学分析，蜡样芽孢杆菌已被分为23种类型，但尚未确定鞭毛的共同抗原决定簇。当免疫化学分类方法从“凝集法”改为“酶联免疫吸附测定（ELISA）”时，各鞭毛类型之间的交叉反应性增加。这些结果表明，ELISA方法的应用将能够检测蜡样芽孢杆菌的共同抗原决定簇。因此，我们尝试制备针对可通过ELISA检测的共同鞭毛抗原的单克隆抗体。所提供的单克隆抗体对蜡样芽孢杆菌（H-1至H-23）具有特异性，与大肠杆菌NIH和枯草芽孢杆菌无交叉反应。
