Yu Zhen-Zhen, Fu Chun-Xiang, Han Ying-Shan, Li Yong-Xing, Zhao De-Xiu
Key Laboratory for Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Beijing 100093, China.
Biotechnol Lett. 2006 Jul;28(13):1027-31. doi: 10.1007/s10529-006-9035-5. Epub 2006 Jun 20.
Addition of 20 muM salicylic acid to Saussurea medusa cell cultures at day 6 resulted in jaceosidin and syringin productions up to 95 mg l(-1 )and 631 mg l(-1) which were, respectively, about 2.5- and 2.7-fold higher than in the control. The biomass was increased from 8 to 12 g l(-1). Expression of chalcone synthase gene (chs) increased sharply after 12 h treatment and was sustained up to 48 h; chalcone isomerase gene (chi) expression reached a peak at 24 h and decreased after 48 h; and phenylalanine ammonia-lyase activity increased by 7.5-fold (96 U mg(-1) protein) higher than in the control after 24 h. These results indicate that salicylic acid enhances the production of jaceosidin and syringin which is accompanied by induction of the related phenylpropanoid biosynthetic enzymes.
在第6天向水母雪莲细胞培养物中添加20μM水杨酸，导致紫铆花素和紫丁香苷的产量分别高达95mg/L和631mg/L，分别比对照高约2.5倍和2.7倍。生物量从8g/L增加到12g/L。查尔酮合酶基因（chs）在处理12小时后表达急剧增加，并持续到48小时；查尔酮异构酶基因（chi）的表达在24小时达到峰值，48小时后下降；苯丙氨酸解氨酶活性在24小时后比对照提高了7.5倍（96U/mg蛋白质）。这些结果表明，水杨酸可提高紫铆花素和紫丁香苷的产量，同时诱导相关苯丙烷生物合成酶。
