Freire Patrick, Vieira Helena L A, Furtado Ana R, de Pedro Miguel A, Arraiano Cecília M
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
FEMS Microbiol Lett. 2006 Jul;260(1):106-11. doi: 10.1111/j.1574-6968.2006.00307.x.
Escherichia coli bolA is a morphogene involved in stress response and cell division. Overexpression of bolA induces biofilm formation and affects the levels of carboxypeptidases PBP5, PBP6 and beta-lactamase AmpC. In this study, we have shown that changes in the expression of bolA result in alterations in the properties of the outer membrane. The sensitivity to detergents and vancomycin was reduced when bolA was overexpressed and fluorescent probes indicated that different levels of bolA had an effect on outer membrane protein accessibility. Moreover, bolA was shown to be involved in the modulation of the OmpF/OmpC balance.
大肠杆菌bolA是一种参与应激反应和细胞分裂的形态发生基因。bolA的过表达会诱导生物膜形成，并影响羧肽酶PBP5、PBP6和β-内酰胺酶AmpC的水平。在本研究中，我们表明bolA表达的变化会导致外膜性质的改变。当bolA过表达时，对去污剂和万古霉素的敏感性降低，荧光探针表明不同水平的bolA对外膜蛋白的可及性有影响。此外，bolA被证明参与了OmpF/OmpC平衡的调节。
