Xiao-Yan Song, Qing-Tao Shen, Shu-Tao Xie, Xiu-Lan Chen, Cai-Yun Sun, Yu-Zhong Zhang
State Key Laboratory of Microbial Technology, Shandong University, Ji'nan, China.
FEMS Microbiol Lett. 2006 Jul;260(1):119-25. doi: 10.1111/j.1574-6968.2006.00316.x.
Antimicrobial metabolites produced by Trichoderma koningii SMF2 exhibited antimicrobial activity against a range of Gram-positive bacterial and fungal phytopathogens. Purification of these metabolites was achieved using combinations of gel filtration and high-performance liquid chromatography. Identified by liquid chromatography electrospray ionization tandem mass spectrometry, the active metabolites proved to be three known peptaibols: Trichokonin VI, VII and VIII. The Trichokonins were stable and remained biological active over a wide pH range and at every temperature tested, showing no loss of activity even after autoclaving. Trichokonins were insensitive to proteolytic enzymes. Trichokonin VI takes on typical helical structure and the structure changes only slightly at different temperatures and pH values. The present study presented the potential of Trichokonins to be used as biological control agents.
康氏木霉SMF2产生的抗微生物代谢产物对一系列革兰氏阳性细菌和真菌植物病原体具有抗菌活性。通过凝胶过滤和高效液相色谱相结合的方法对这些代谢产物进行了纯化。经液相色谱电喷雾电离串联质谱鉴定，活性代谢产物为三种已知的肽菌素：抗霉菌素VI、VII和VIII。抗霉菌素在很宽的pH范围内以及在每个测试温度下都很稳定并保持生物活性，即使经过高压灭菌也没有活性损失。抗霉菌素对蛋白水解酶不敏感。抗霉菌素VI具有典型的螺旋结构，并且在不同温度和pH值下结构仅略有变化。本研究展示了抗霉菌素用作生物防治剂的潜力。
