Suri C, Fung B P, Tischler A S, Chikaraishi D M
Neuroscience Program, Tufts University School of Medicine, Boston, Massachusetts 02111.
J Neurosci. 1993 Mar;13(3):1280-91. doi: 10.1523/JNEUROSCI.13-03-01280.1993.
Brain (CATH.a) and adrenal (PATH.1 and PATH.2) cell lines have been established that synthesize abundant dopamine and norepinephrine and express the appropriate catecholaminergic biosynthetic enzymes, tyrosine hydroxylase (TH) and dopamine beta-hydroxylase. The lines were derived from TH-positive tumors in transgenic mice carrying the SV40 T antigen oncogene under the transcriptional control of 773 base pairs of 5' flanking sequences from the rat TH gene. Although the lines continue to express T antigen, they exhibit neuronal properties such as neurofilaments and synaptophysin and lack glial intermediate filaments. Although in vivo TH is only expressed in postmitotic neurons in the CNS, the CATH.a line demonstrates that TH expression and continued cell division are not incompatible after oncogenic transformation.
已建立脑（CATH.a）和肾上腺（PATH.1和PATH.2）细胞系，这些细胞系能合成大量多巴胺和去甲肾上腺素，并表达适当的儿茶酚胺能生物合成酶，即酪氨酸羟化酶（TH）和多巴胺β-羟化酶。这些细胞系源自携带SV40 T抗原癌基因的转基因小鼠中的TH阳性肿瘤，该癌基因受大鼠TH基因5'侧翼序列773个碱基对的转录控制。尽管这些细胞系继续表达T抗原，但它们表现出神经元特性，如神经丝和突触素，并且缺乏胶质中间丝。尽管在体内TH仅在中枢神经系统的有丝分裂后神经元中表达，但CATH.a细胞系表明致癌转化后TH表达和细胞持续分裂并非不相容。
