O'Keefe J S, Murray A, Wilks C R, Moriarty K M
Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand.
Res Vet Sci. 1991 May;50(3):349-51. doi: 10.1016/0034-5288(91)90137-d.
Unpurified DNA derived from cultures of equine fetal kidney cells infected with either equine herpesvirus type 1 or equine herpesvirus type 4 was amplified by the polymerase chain reaction using one pair of oligonucleotide primers. Restriction endonuclease digestion of the amplified segments with PvuII, followed by electrophoresis, revealed restriction fragment length polymorphisms which enabled the two virus types to be differentiated.
用一对寡核苷酸引物通过聚合酶链反应扩增来自感染1型马疱疹病毒或4型马疱疹病毒的马胎儿肾细胞培养物的未纯化DNA。用PvuII对扩增片段进行限制性内切酶消化，然后进行电泳，显示出限制性片段长度多态性，从而能够区分这两种病毒类型。
