Joseph Joveeta, Vemuganti Geeta K, Garg Prashant, Sharma Savitri
Jhaveri Microbiology Centre, Hyderabad Eye Research Foundation, L.V.Prasad Eye Institute, L.V.Prasad Marg, Banjara Hills, Hyderabad-500 034, India.
BMC Clin Pathol. 2006 Jun 23;6:6. doi: 10.1186/1472-6890-6-6.
There is limited data on comparing stains in the detection of microsporidia in corneal biopsies. Hence we wanted to evaluate various stains for their ability to detect microsporidia in corneal tissue sections.
Four cases diagnosed with microsporidiosis on Hematoxylin and Eosin and Periodic Acid Schiff's stained sections of the corneal button between January 2002 and December 2004, were included. Further sections were prospectively stained with calcofluor white, Gram, Giemsa, Masson's trichrome, acridine orange, Gomori's methenamine silver, Gram's chromotrope and modified acid fast stain. The stained sections were analyzed for the spore characteristics in terms of size, shape, color contrast, cell wall morphology, waist band in cytoplasm and ease of detection.
All sections showed microsporidial spores as 3-5 microm, oval bodies. 1% acid fast, Gram's chromotrope and GMS stains provided a reliable diagnosis of microsporidia as diagnostic waist band could be identified and good contrast helped distinguish the spores from inflammatory debris.
Considering the ease of performance, cost effectiveness and rapidity of the technique, 1% acid fast stain and Gram's chromotrope stain are ideal for the detection of microsporidia.
在角膜活检中比较不同染色剂检测微孢子虫的数据有限。因此，我们想评估各种染色剂在角膜组织切片中检测微孢子虫的能力。
纳入2002年1月至2004年12月间经苏木精-伊红染色和过碘酸希夫染色的角膜纽扣标本诊断为微孢子虫病的4例病例。进一步的切片前瞻性地用荧光钙白、革兰氏染色、吉姆萨染色、马森三色染色、吖啶橙、戈莫里六胺银染色、革兰氏铬变素染色和改良抗酸染色。对染色切片的孢子特征进行分析，包括大小、形状、颜色对比、细胞壁形态、细胞质中的腰带以及检测的难易程度。
所有切片均显示微孢子虫孢子为3 - 5微米的椭圆形体。1%抗酸染色、革兰氏铬变素染色和六胺银染色能可靠诊断微孢子虫，因为可识别出诊断性腰带，且良好的对比度有助于将孢子与炎性碎屑区分开来。
考虑到操作的简便性、成本效益和技术的快速性，1%抗酸染色和革兰氏铬变素染色是检测微孢子虫的理想方法。
