Herrero Ana, Padrós Francesc, Pflaum Sara, Matthews Chris, Del-Pozo Jorge, Rodger Hamish D, Dagleish Mark P, Thompson Kim D
Moredun Research Institute, Pentlands Science Park, Scotland, UK (Herrero, Dagleish, Thompson).
Unitat de Biologia, Facultat de Veterinària, Universitat Autònoma de Barcelona, Barcelona, Catalunya, Spain (Padrós).
J Vet Diagn Invest. 2020 Jan;32(1):142-146. doi: 10.1177/1040638719887707. Epub 2019 Nov 18.
is a microsporidian associated with gill disease in farmed Atlantic salmon (). Detection of the parasite in histologic tissue sections is challenging using common histochemical stains given that the small, widely distributed parasite spores typically occur individually or in small clusters. We compared the ability of 4 histologic methods to detect spores in serial sections of Atlantic salmon gill tissue: hematoxylin and eosin (H&E), Gram-Twort (GT), calcofluor white (CW), and immunohistochemistry (IHC). Using CW as a benchmark to calculate a relative ratio, IHC consistently detected more spores than CW (median: 1.3), followed by GT (median: 0.2) and H&E (median: 0.1). IHC detected significantly more spores than GT ( < 0.05) and H&E ( < 0.05), and GT more than H&E ( < 0.05). We found significant underestimation of numbers of microsporidia spores in gill disease in Atlantic salmon using conventional histochemical stains and recommend the use of CW or IHC to detect the parasite in tissue sections.
是一种与养殖大西洋鲑鱼鳃病相关的微孢子虫（）。鉴于这种小而广泛分布的寄生虫孢子通常单个或成小簇出现，使用普通组织化学染色在组织学切片中检测该寄生虫具有挑战性。我们比较了4种组织学方法在大西洋鲑鱼鳃组织连续切片中检测孢子的能力：苏木精和伊红染色（H&E）、革兰氏-特沃特染色（GT）、荧光增白剂染色（CW）和免疫组织化学染色（IHC）。以CW作为计算相对比率的基准，IHC始终比CW检测到更多的孢子（中位数：1.3），其次是GT（中位数：0.2）和H&E（中位数：0.1）。IHC检测到的孢子明显多于GT（<0.05）和H&E（<0.05），且GT多于H&E（<0.05）。我们发现，使用传统组织化学染色会显著低估大西洋鲑鱼鳃病中微孢子虫孢子的数量，并建议使用CW或IHC来检测组织切片中的寄生虫。
