Rosenberg N, Schaeffer W I, Diamond L
Cancer Res. 1975 Aug;35(8):1970-4.
Variant cell lines that are sensitive to density-dependent inhibition of growth have been isolated from three of four SV40-transformed human fibroblast cell lines. Variants were isolated by plating the transformed cells at low density, by treating them with 5-fluorodeoxyuridine, or by growing them on glutaraldehyde-fixed monolayers of normal cells. The variant cell lines, isolated at a frequency of about 2% of all cells forming colonies after treatment, were initially recognized by colonial morphology, and the variant pheno-types were confirmed, after subculturing, by saturation-density determinations. The variant cell lines reach saturation densities that are 40% or less than those of the parent cell lines, and plate in soft agar medium at reduced efficiency, compared with the parent cells. They retain SV40 T antigen. The modal chromosome numbers of two of the variant cell lines were increased, compared with those of the parent cell lines; two other variants were indistinguishable in chromosome number from the parent cells. Stability of these properties over a 6-month period was demonstrated with two of the variants.
已从4株SV40转化的人成纤维细胞系中的3株中分离出对密度依赖性生长抑制敏感的变异细胞系。通过将转化细胞低密度接种、用5-氟脱氧尿苷处理或在正常细胞的戊二醛固定单层上培养来分离变异株。变异细胞系的分离频率约为处理后形成集落的所有细胞的2%，最初通过集落形态识别，传代培养后通过饱和密度测定确认变异表型。与亲代细胞系相比，变异细胞系达到的饱和密度是亲代细胞系的40%或更低，并且在软琼脂培养基中接种的效率降低。它们保留了SV40 T抗原。与亲代细胞系相比，其中两个变异细胞系的众数染色体数增加；另外两个变异株的染色体数与亲代细胞无法区分。其中两个变异株在6个月期间证明了这些特性的稳定性。
