Ruben R L, Rafferty K A
J Natl Cancer Inst. 1978 Oct;61(4):993-1000.
A simian virus 40 (SV40)-transformed line of human parapharyngeal cells (SV-TGo) was cultured in semisolid agar to determine its ability to grow in the absence of an anchoring substratum and to evaluate any phenotypic changes that might have resulted during the isolation of sublines specifically selected for anchorage independence. After 2--3 weeks and 14--15 population doublings in culture, SV-TGo plated with over 1,000% higher efficiency than negative controls (F2408 cells). Sublines, 0.3--2.0 mm in diameter, were isolated and transferred to Leighton tubes in which they underwent an additional 0--7 divisions before senescence after 39--44 total population doublings. Subline phenotype was identical to the original parental phenotype, including epithelioid morphology, organized pattern of growth, extreme sensitivity to density-dependent inhibition of growth, and continuous production of infectious SV40 as detected by the combined tests of cocultivation and direct isolation. Limited division potential was within the range observed for the parental line. The ability to grow in agar without identifiable phenotypic changes was therefore confirmed for this line of SV40-transformed human epithelioid cells.
将一株猿猴病毒40（SV40）转化的人咽旁细胞系（SV-TGo）培养于半固体琼脂中，以确定其在无锚定基质情况下的生长能力，并评估在分离特别选择的非锚定依赖性亚系过程中可能产生的任何表型变化。在培养2 - 3周及经历14 - 15次群体倍增后，SV-TGo的接种效率比阴性对照（F2408细胞）高出1000%以上。分离出直径为0.3 - 2.0毫米的亚系，并转移至莱顿管中，在总共39 - 44次群体倍增后衰老前，它们在其中又经历了0 - 7次分裂。亚系的表型与原始亲本表型相同，包括上皮样形态、有组织的生长模式、对密度依赖性生长抑制极度敏感，以及通过共培养和直接分离的联合试验检测到持续产生传染性SV40。有限的分裂潜能在亲代细胞系观察到的范围内。因此，对于这株SV40转化的人上皮样细胞系，证实了其在琼脂中生长且无明显表型变化的能力。
