Leyden J J, McGinley K J, Kaminer M S, Bakel J, Nishijima S, Grove M J, Grove G L
University of Pennsylvania, School of Medicine, Department of Dermatology, Philadelphia 19104.
J Hosp Infect. 1991 Jun;18 Suppl B:13-22. doi: 10.1016/0195-6701(91)90258-a.
A method is described for quantification of the bacterial flora on the hand surface. Computer-assisted image analysis of bacterial growth of large full-hand touch plates provides a quantifiable measure of the bacterial flora on the hand surface. Image analysis pixel intensity values showed a significant correlation (P less than 0.0001) with colony forming unit values determined by the glove juice method. Image analysis of impressions from hands treated with various antimicrobial agents in detergent bases showed that 4% chlorhexidine gluconate produces a 96% reduction after a 30 s washing and 98% reduction after a 3 min washing while 7.5% povidone-iodine and 1% triclosan produce a 77% and 70% reduction after 3 min respectively, and 70% isopropanol produces a 98% reduction after a 30 s wash.
本文描述了一种对手表面细菌菌群进行定量的方法。通过计算机辅助图像分析大型全手触摸平板上的细菌生长情况，可提供手表面细菌菌群的可量化指标。图像分析像素强度值与通过手套汁液法测定的菌落形成单位值显示出显著相关性（P小于0.0001）。对用含洗涤剂基质的各种抗菌剂处理过的手部印记进行图像分析表明，4%葡萄糖酸氯己定在洗涤30秒后细菌减少96%，洗涤3分钟后减少98%；而7.5%聚维酮碘和1%三氯生在3分钟后分别减少77%和70%；70%异丙醇在洗涤30秒后减少98%。
