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从一次性尿布中收集尿液。

Urine collection from disposable nappies.

作者信息

Ahmad T, Vickers D, Campbell S, Coulthard M G, Pedler S

机构信息

Department of Child Health, Royal Victoria Infirmary, Newcastle upon Tyne, UK.

出版信息

Lancet. 1991 Sep 14;338(8768):674-6. doi: 10.1016/0140-6736(91)91242-m.

Abstract

Urine was collected for microscopy and culture by standard use of sterile adhesive bags and by extraction from wet disposable nappies from 45 patients aged 1 to 23 months. Urine can readily be obtained by compression of wet nappy fibres within a 20 ml syringe if highly absorbent brands that contain gel beads are avoided. Red and white cell numbers were reduced on light microscopy of specimens obtained from nappies, but bacterial counts were unchanged. On culture, 6 children were shown to have urinary tract infections by both methods; of the children who did not have a urine infection, the contamination rate was lower from nappy collections (10/39 vs 17/39 from urine bags). Biochemical analysis of urine collected from 11 older children showed very close correlation for sodium, potassium, urea, and creatinine concentrations and osmolality between freshly voided urine and samples obtained after they were soaked into disposable nappies for 3 h and reclaimed, although there was greater variation for measurements of calcium and phosphate. Extraction of urine from disposable nappies put on within 4 h and which are not soiled by faeces is an inexpensive, rapid, and simple method to collect urine from young children for culture, microscopy for bacteria, and biochemical analysis.

摘要

采用标准方法,使用无菌粘贴袋并从45名年龄在1至23个月的患者的湿一次性尿布中提取尿液,用于显微镜检查和培养。如果避免使用含有凝胶珠的高吸水性品牌尿布,通过在20毫升注射器中挤压湿尿布纤维就可以轻松获取尿液。从尿布中获取的标本在光学显微镜下红细胞和白细胞数量减少,但细菌计数不变。培养结果显示,两种方法均表明6名儿童患有尿路感染;在没有尿路感染的儿童中,从尿布收集尿液的污染率较低(10/39,而从尿袋收集尿液的污染率为17/39)。对11名年龄较大儿童收集的尿液进行生化分析表明,新鲜排出尿液与浸泡在一次性尿布中3小时后回收的样本之间,钠、钾、尿素、肌酐浓度和渗透压具有非常密切的相关性,尽管钙和磷的测量值变化较大。从4小时内使用且未被粪便污染的一次性尿布中提取尿液,是一种廉价、快速且简单的方法,可用于收集幼儿尿液以进行培养、细菌显微镜检查和生化分析。

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