消毒剂杀结核杆菌试验的标准化

Standardization of tuberculocidal testing of disinfectants.

作者信息

Ascenzi J M

机构信息

Johnson and Johnson Medical, Inc., Arlington, TX 76004-0130.

出版信息

J Hosp Infect. 1991 Jun;18 Suppl A:256-63. doi: 10.1016/0195-6701(91)90031-3.

DOI:10.1016/0195-6701(91)90031-3

PMID:1679791

Abstract

The current AOAC tuberculocidal test has poor reproductibility caused by growth conditions of the test culture, lack of standardization of the challenge inoculum, poor growth in the recovery medium and the use of carriers. A procedure for growth of Mycobacterium bovis BCG is presented which yields cultures that can be easily standardized. Among currently used recovery media, Proskauer-Beck broth does not allow recovery of bovis BCG when 10 cfu or less are present and Middlebrook 7H9 broth provides the best recovery of cells.

摘要

当前的美国分析化学家协会（AOAC）杀结核菌试验的重现性较差，这是由试验培养物的生长条件、挑战接种物缺乏标准化、在复苏培养基中生长不佳以及载体的使用所导致的。本文介绍了一种牛分枝杆菌卡介苗（Mycobacterium bovis BCG）的培养方法，该方法能产生易于标准化的培养物。在目前使用的复苏培养基中，当存在10个或更少的菌落形成单位（cfu）时，普罗斯考尔-贝克肉汤（Proskauer-Beck broth）无法使牛分枝杆菌卡介苗复苏，而Middlebrook 7H9肉汤能实现最佳的细胞复苏效果。