Wortzman Genevieve B, Gardner Allison J, Evans Janice P
Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Johns Hopkins University of Bloomberg School of Public Health, Baltimore, MD, USA.
Methods Mol Biol. 2006;341:89-101. doi: 10.1385/1-59745-113-4:89.
The interactions between egg and sperm are among the most fascinating in cell biology. These interactions include cell-cell adhesion and then membrane fusion between the two gametes. This chapter details the experimental methods used to for gamete culture and in vitro fertilization using mouse sperm and eggs.
卵子与精子之间的相互作用是细胞生物学中最引人入胜的内容之一。这些相互作用包括细胞间黏附，然后是两个配子之间的膜融合。本章详细介绍了使用小鼠精子和卵子进行配子培养和体外受精的实验方法。
