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用于人类精卵相互作用的细胞表面蛋白质网络的构建与分析

Construction and Analysis of the Cell Surface's Protein Network for Human Sperm-Egg Interaction.

作者信息

Fard Jahromi Soudabeh Sabetian, Shamsir Mohd Shahir

机构信息

Department of Biological and Health Sciences, Faculty of Bioscience & Medical Engineering, Universiti Teknologi Malaysia, 81310 Johor, Malaysia.

出版信息

ISRN Bioinform. 2013 Aug 12;2013:962760. doi: 10.1155/2013/962760. eCollection 2013.

DOI:10.1155/2013/962760
PMID:25937952
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4393059/
Abstract

Sperm-egg interaction is one of the most impressive processes in sexual reproduction, and understanding the molecular mechanism is crucial in solving problems in infertility and failed in vitro fertilization. The main purpose of this study is to map the sperm-egg interaction network between cell-surface proteins and perform an interaction analysis on this new network. We built the first protein interaction network of human sperm-egg binding and fusion proteins that consists of 84 protein nodes and 112 interactions. The gene ontology analysis identified a number of functional clusters that may be involved in the sperm-egg interaction. These include G-protein coupled receptor protein signaling pathway, cellular membrane fusion, and single fertilization. The PPI network showed a highly interconnected network and identified a set of candidate proteins: ADAM-ZP3, ZP3-CLGN, IZUMO1-CD9, and ADAM2-IZUMO1 that may have an important role in sperm-egg interaction. The result showed that the ADAM2 may mediate interaction between two essential factors CD9 and IZUMO1. The KEGG analysis showed 12 statistically significant pathways with 10 proteins associated with cancer, suggesting a common pathway between tumor fusion and sperm-egg fusion. We believe that the availability of this map will assist future researches in the fertilization mechanism and will also facilitate biological interpretation of sperm-egg interaction.

摘要

精卵相互作用是有性生殖中最引人注目的过程之一，了解其分子机制对于解决不孕症和体外受精失败问题至关重要。本研究的主要目的是绘制细胞表面蛋白之间的精卵相互作用网络，并对这个新网络进行相互作用分析。我们构建了第一个由84个蛋白节点和112个相互作用组成的人类精卵结合与融合蛋白的蛋白相互作用网络。基因本体分析确定了一些可能参与精卵相互作用的功能簇。这些包括G蛋白偶联受体蛋白信号通路、细胞膜融合和单受精。蛋白质-蛋白质相互作用（PPI）网络显示出一个高度互联的网络，并确定了一组候选蛋白：ADAM-ZP3、ZP3-CLGN、IZUMO1-CD9和ADAM2-IZUMO1，它们可能在精卵相互作用中起重要作用。结果表明，ADAM2可能介导两个关键因子CD9和IZUMO1之间的相互作用。京都基因与基因组百科全书（KEGG）分析显示有12条具有统计学意义的通路，其中10种蛋白与癌症相关，这表明肿瘤融合和精卵融合之间存在共同通路。我们相信，这一图谱的可用性将有助于未来对受精机制的研究，也将促进对精卵相互作用的生物学解读。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08f6/4393059/a6a3660ddd9f/ISRN.BIOINFORMATICS2013-962760.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08f6/4393059/1934bc50b8b9/ISRN.BIOINFORMATICS2013-962760.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08f6/4393059/f9df3fa89370/ISRN.BIOINFORMATICS2013-962760.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08f6/4393059/f6b390a3a959/ISRN.BIOINFORMATICS2013-962760.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08f6/4393059/0f896381825a/ISRN.BIOINFORMATICS2013-962760.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08f6/4393059/a6a3660ddd9f/ISRN.BIOINFORMATICS2013-962760.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08f6/4393059/1934bc50b8b9/ISRN.BIOINFORMATICS2013-962760.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08f6/4393059/f9df3fa89370/ISRN.BIOINFORMATICS2013-962760.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08f6/4393059/f6b390a3a959/ISRN.BIOINFORMATICS2013-962760.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08f6/4393059/0f896381825a/ISRN.BIOINFORMATICS2013-962760.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08f6/4393059/a6a3660ddd9f/ISRN.BIOINFORMATICS2013-962760.005.jpg

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PLoS One. 2012;7(9):e44548. doi: 10.1371/journal.pone.0044548. Epub 2012 Sep 5.
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Protease activity involvement in the passage of mammalian sperm through the zona pellucida.蛋白酶活性在哺乳动物精子穿过透明带过程中的作用。
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不同必需性对人类睾丸蛋白序列进化的影响。
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