Hreggvidsson Gudmundur O, Skirnisdottir Sigurlaug, Smit Bart, Hjorleifsdottir Sigridur, Marteinsson Viggo Th, Petursdottir Solveig, Kristjansson Jakob K
Prokaria Ltd., Gylfaflot 5, IS-112 Reykjavik, Iceland.
Extremophiles. 2006 Dec;10(6):563-75. doi: 10.1007/s00792-006-0530-3. Epub 2006 Jun 24.
Genetic relationships and diversity of 101 Thermus isolates from different geothermal regions in Iceland were investigated by using multilocus enzyme electrophoresis (MLEE) and small subunit ribosomal rRNA (SSU rRNA) sequence analysis. Ten polymorphic enzymes were used and seven distinct and genetically highly divergent lineages of Thermus were observed. Six of seven lineages could be assigned to species whose names have been validated. The most diverse lineage was Thermus scotoductus. In contrast to the other lineages, this lineage was divided into very distinct genetic sublineages that may represent subspecies with different habitat preferences. The least diverse lineage was Thermus brockianus. Phenotypic and physiological analysis was carried out on a subset of the isolates. No relationship was found between growth on specific single carbon source to the grouping obtained by the isoenzyme analysis. The response to various salts was distinguishing in a few cases. No relationship was found between temperature at the isolation site and the different lineages, but pH indicated a relation to specific lineages.
通过多位点酶电泳（MLEE）和小亚基核糖体RNA（SSU rRNA）序列分析，研究了来自冰岛不同地热区域的101株嗜热栖热菌的遗传关系和多样性。使用了10种多态性酶，观察到嗜热栖热菌的7个不同且遗传上高度分化的谱系。7个谱系中的6个可以归为已被验证名称的物种。最多样化的谱系是嗜热栖热菌。与其他谱系不同，该谱系被分为非常不同的遗传亚谱系，可能代表具有不同栖息地偏好的亚种。最不多样化的谱系是布氏嗜热栖热菌。对一部分分离株进行了表型和生理分析。在特定单一碳源上的生长与同工酶分析得到的分组之间未发现相关性。在少数情况下，对各种盐的反应具有区分性。在分离位点的温度与不同谱系之间未发现相关性，但pH值表明与特定谱系有关。
