Bharucha K, Kulkarni S, Nair S, Ghosh K, Mohanty D, Walvekar V, Raut V
Institute of Immunohaematology, KEM Hospital Campus, Parel, Mumbai 400012, India.
Platelets. 1999 Jul;10(4):197-202. doi: 10.1080/09537109976022.
It is believed that platelets play a key role in the production of pre-eclamptic toxaemia and toxaemia of pregnancy. Toxaemia of pregnancy is described as a condition of chronic DIC where there is thrombocytopenia as well as raised fibrin degradation products. Since fibrinogen receptors are involved in the final stage of the platelet aggregation reaction, we wanted to investigate the platelet receptors for fibrinogen in normal and abnormal pregnancy. Thirty-six normal pregnant women (12 in their 2nd trimester, 24 in their 3rd trimester), 24 pregnant pre-eclamptic toxaemia cases and 16 non-pregnant controls were included in the present study. All patients with pre-eclamptic toxaemia had oedema, proteinuria and hypertension. Flow cytometric study of platelets was undertaken utilizing fluorescein isothiocyanate (FITC)-labelled anti-human fibrinogen antibody in unstimulated and ADP-stimulated (final concentration 0.02 M) platelets. The intensity of platelet fluorescence was classified into three groups and expressed in arbitrary units. The results indicate that there are a higher number of stimulated platelets expressing fibrinogen receptors in the circulation of patients with pre-eclampsia. Thus, it is possible to hypothesize that platelets showing increased fibrinogen receptors aggregate and form microthrombi in smaller vessels in women with pre-eclamptic toxaemia.
人们认为血小板在子痫前期毒血症和妊娠毒血症的产生中起关键作用。妊娠毒血症被描述为一种慢性弥散性血管内凝血状态，存在血小板减少以及纤维蛋白降解产物升高的情况。由于纤维蛋白原受体参与血小板聚集反应的最后阶段，我们想要研究正常妊娠和异常妊娠中血小板的纤维蛋白原受体。本研究纳入了36名正常孕妇（12名处于孕中期，24名处于孕晚期）、24例子痫前期毒血症孕妇病例以及16名非孕对照者。所有子痫前期毒血症患者均有水肿、蛋白尿和高血压。利用异硫氰酸荧光素（FITC）标记的抗人纤维蛋白原抗体对未刺激和ADP刺激（终浓度0.02M）的血小板进行血小板的流式细胞术研究。血小板荧光强度分为三组并以任意单位表示。结果表明，子痫前期患者循环中表达纤维蛋白原受体的受刺激血小板数量更多。因此，可以假设在子痫前期毒血症女性中，显示纤维蛋白原受体增加的血小板聚集并在较小血管中形成微血栓。
